Membrane ultrafilter Vivaspin® 6

for liquidsfor protein solutionsfor serum

Product overview
Vivaspin® 6 centrifugal concentrators enable rapid concentration of protein and nanoparticle samples up to 6 ml. They fit rotors that accept 15 ml (17 mm) conical bottom tubes. The patented twin vertical polyethersulfone (PES) membrane design reduces membrane fouling and shortens spin times. A built-in dead stop pocket improves concentrate recovery.

Key features

• Model: Vivaspin® 6
• Maximum start volume: 6 ml
• Concentration factor: up to 200x
• Patented twin vertical PES membranes to reduce fouling and speed spins
• Dead stop pocket for maximum concentrate recovery (30 µl dead stop volume)
• Compatible with rotors accepting 15 ml (17 mm) conical bottom tubes
• Multiple MWCO options available (PES)

Applications

• Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis
• Concentration of cerebrospinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis
• Protein removal from serum and cell/tissue lysates
• Buffer exchange for proteomic peptide samples
• Sample preparation for mass spectrometry

Performance (time to concentrate up to 30x at 20°C and solute recovery %)

• Cytochrome c 0.25 mg/ml (12,400 MW) — 5,000 MWCO PES: Swing bucket (3,000 x g) Min: — ; Rec.: — ; Fixed angle 25° (7,500 x g) Min: 90 ; Rec.: 97%
• BSA 1.0 mg/ml (66,000 MW) — 5,000 MWCO PES: Swing bucket (3,000 x g) Min: 20 ; Rec.: 98% ; Fixed angle 25° (7,500 x g) Min: 12 ; Rec.: 98%
• BSA — 10,000 MWCO PES: Swing bucket Min: 13 ; Rec.: 98% ; Fixed angle Min: 10 ; Rec.: 98%
• BSA — 30,000 MWCO PES: Swing bucket Min: 12 ; Rec.: 98% ; Fixed angle Min: 9 ; Rec.: 97%
• IgG 0.25 mg/ml (160,000 MW) — 30,000 MWCO PES: Swing bucket Min: 18 ; Rec.: 96% ; Fixed angle Min: 15 ; Rec.: 95%
• IgG — 50,000 MWCO PES: Swing bucket Min: 17 ; Rec.: 96% ; Fixed angle Min: 14 ; Rec.: 96%
• IgG — 100,000 MWCO PES: Swing bucket Min: 15 ; Rec.: 91% ; Fixed angle Min: 12 ; Rec.: 91%
• Latex beads 0.004% (0.055 µm) — 300,000 MWCO PES: Swing bucket Min: — ; Rec.: — ; Fixed angle Min: 25 ; Rec.: 99%
• Latex beads 0.004% (0.24 µm) — 1,000,000 MWCO PES: Swing bucket Min: — ; Rec.: — ; Fixed angle Min: 4 ; Rec.: 99%
• Yeast 1.0 mg/ml (S. cerevisiae) — 0.2 µm PES: Swing bucket Min: 4 ; Rec.: 97% ; Fixed angle Min: 3 ; Rec.: 97%

Technical specifications

• Concentrator capacity: Swing bucket rotor 6 ml; Fixed angle rotor 6 ml
• Dimensions: Length 122 mm; Width 17 mm
• Active membrane area: 2.5 cm²
• Hold-up volume (membrane and support): <10 µl
• Dead stop volume: 30 µl
• Materials: Body and filtrate vessel polycarbonate; Cap polypropylene; Membrane polyethersulfone (PES)
• Compatible tube size: fits 15 ml (17 mm) conical bottom tubes
• Recommended maximum g-force: swing bucket up to 4,000 g; fixed angle up to 8,000 g (note: devices with membrane MWCO ≥ 100 kDa may require lower g forces)

Equipment required

• Recommended rotor types: swing bucket or fixed angle (minimum fixed angle 25°)
• Rotor cavity: must accept 15 ml (17 mm) conical bottom tubes
• Maximum speeds: swing bucket up to 4,000 g; fixed angle up to 8,000 g (adjust for high MWCO as noted)

Ordering & packaging examples

• Available MWCO options (examples): 3,000; 5,000; 10,000; 30,000; 50,000; 100,000; 300,000; 1,000,000 MWCO PES; 0.2 µm PES
• Pack sizes: typical pack options include 25 or 100 per pack (0.2 µm available in 100 per pack)
• Prices vary by SKU and pack size; contact supplier for full ordering information