Membrane ultrafilter Vivaspin® 4 Turbo

for liquidsfor protein solutionsfor serum

Summary
Vivaspin® Turbo 4 centrifugal concentrators accept up to 4 ml starting volume and enable rapid concentration of protein and nanoparticle samples up to 100×. The patented twin-vertical PES membrane design reduces membrane fouling and provides fast spin times. Compatible with swing-bucket rotors that accept 15 ml centrifuge tubes. A built-in dead-stop pocket enables maximal, pipette-friendly concentrate recovery.

Applications

• Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
• Concentration of cerebrospinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
• Protein removal from serum and from cell or tissue lysates.
• Buffer exchange for proteomic peptide samples.
• Sample preparation for mass spectrometry.

Performance
Rotor type: Swing-bucket. Recommended centrifugal force: 4,000 x g. Starting volume: 4 ml.
BSA 1.0 mg/ml (66,000 Da): 10,000 MWCO PES — Time: 10 min — Concentrate recovery: 98%
30,000 MWCO PES — Time: 8 min — Concentrate recovery: 96%
IgG 0.25 mg/ml (160,000 Da): 30,000 MWCO PES — Time: 18 min — Concentrate recovery: 94%
50,000 MWCO PES — Time: 16 min — Concentrate recovery: 93%
100,000 MWCO PES — Time: 17 min — Concentrate recovery: 94%
Note: 100,000 MWCO data generated at lower centrifugal force (3,000 x g).

Ordering options (membrane MWCO and pack sizes)
Available membrane MWCOs (PES): 3,000; 5,000; 10,000; 30,000; 50,000; 100,000. Pack sizes: 25 per pack or 100 per pack.

Technical specifications

• Concentrator capacity: Swing-bucket rotor — 4 ml; Fixed-angle rotor — 4 ml.
• Dimensions: Total length — 122 mm; Width — 14.6 mm.
• Active membrane area: 3.2 cm².
• Hold-up volume of membrane: <10 µl.
• Dead-stop volume: Swing-bucket — 40 µl; Fixed-angle — 30 µl.
• Materials: Body — styrene butadiene copolymer; Filtrate vessel — polypropylene; Concentrator cap — polypropylene; Membrane — polyethersulfone (PES).
• Maximum speed: Swing-bucket — 4,000 x g; Fixed-angle — up to 7,500 x g (devices with membrane MWCO ≥ 100 kDa must be processed at lower g).
• Rotor cavity: Fits 15 ml (17 mm) conical-bottom tubes.
• Minimum fixed-rotor angle: 25°.
• Concentrate recovery: Compatible with fixed or variable-volume pipettes; recommended tip type — thin gel-loader style.