Membrane ultrafilter Vivaspin® 100

for liquidsfor protein solutionslaboratory

Product overview
Vivaspin® 100 centrifugal concentrators enable rapid concentration of protein and nanoparticle samples up to 98 ml. Compatible with swing-bucket rotors that accept 250 ml centrifuge bottles or with a pressure head for pressure-facilitated filtration. The patented twin-vertical PES membrane design reduces filter fouling and provides fast spin times. Built-in dead-stop pocket maximizes concentrate recovery. Available operation modes: centrifugation, pressure-fugation or gas pressure filtration. Concentrate up to 28×.

Performance (time to concentrate up to 30× [min] at 20°C — 90 ml start volume)

• BSA 1.0 mg/ml (66,000 MW) — 5,000 MWCO PES | No agitation: 22 | Orbital shake: 75 | As pressure cell (4 bar / 60 psi): 25 | Solute recovery: 96%
• BSA 1.0 mg/ml (66,000 MW) — 10,000 MWCO PES | No agitation: 16 | Orbital shake: 60 | As pressure cell (4 bar / 60 psi): 20 | Solute recovery: 96%
• BSA 1.0 mg/ml (66,000 MW) — 30,000 MWCO PES | No agitation: 16 | Orbital shake: 60 | As pressure cell (4 bar / 60 psi): 20 | Solute recovery: 94%
• IgG 0.25 mg/ml (160,000 MW) — 50,000 MWCO PES | No agitation: 20 | Orbital shake: 70 | As pressure cell (4 bar / 60 psi): 30 | Solute recovery: 94%
• IgG 0.25 mg/ml (160,000 MW) — 100,000 MWCO PES | No agitation: 20 | Orbital shake: 85 | As pressure cell (4 bar / 60 psi): 30 | Solute recovery: 90%
• Latex beads 0.004% in DMEM +10% FCS (0.055 µm) — 300,000 MWCO PES | No agitation: 35 | Orbital shake: - | As pressure cell (4 bar / 60 psi): 120 | Solute recovery: 99%
• Latex beads 0.004% in DMEM +10% FCS (0.24 µm) — 1,000,000 MWCO PES* | No agitation: 4 | Orbital shake: 5 | As pressure cell (4 bar / 60 psi): 4 | Solute recovery: 99%

Note: *2,000 g in centrifuge, 2 bar (29 psi).

Available pore sizes and membrane types

• Polyethersulfone (PES) membranes
• Pore / MWCO options: 0.2 µm; 5,000 MWCO; 10,000 MWCO; 30,000 MWCO; 50,000 MWCO; 100,000 MWCO; 300,000 MWCO; 1,000,000 MWCO

Pack sizes / ordering formats

• 2 per pack
• 10 per pack

Accessories (examples)

• Air Pressure Controller (APC)
• Pressure head for Vivaspin 100 with replacement seals
• Charge valve for pressure head
• Female and male connectors
• 4 mm OD pneumatic tube (3 ml)
• Vivaspin 20 pressure head
• Diafiltration cups (12 per pack)

Technical specifications

• Concentrator capacity (swing bucket rotor): 90 ml
• Concentrator capacity (with pressure head): 98 ml
• Total length: 123 mm (centrifugal)
• Total length with pressure head: 197 mm
• Width: 62 mm
• Active membrane area: 23.5 cm²
• Hold-up volume of membrane: <250 µl
• Dead stop volume: 350 µl
• Rotor type: Swing bucket
• Rotor cavity: To fit 250 ml (62 mm) centrifuge bottles (maximum cavity depth 105 mm)
• Maximum speed: 2,000 g
• Maximum pressure: 5 bar (75 psi)
• Body material: Polycarbonate
• Filtrate vessel material: Polycarbonate
• Concentrator cap material: Santoprene
• Pressure head material: Acetal
• Membrane material: Polyethersulfone (PES)