Membrane ultrafilter Vivaspin® 15R

for liquidsfor protein solutionsfor serum

Product overview
Vivaspin® 15R centrifugal devices enable rapid concentration of protein and nanoparticle samples from starting volumes up to 15 ml. Compatible with rotors that accept 50 ml centrifuge tubes. The patented twin vertical membrane design reduces filter fouling while the Hydrosart® regenerated cellulose membrane provides high recovery for antibodies and dilute protein samples. A built-in dead stop pocket allows maximum concentrate recovery.

Key performance summary

• Starting volume: up to 15 ml; concentration factors up to ~400x depending on membrane MWCO and rotor conditions.
• Membrane options: Hydrosart® MWCOs 2,000; 5,000; 10,000; 30,000. Cellulose triacetate 20,000 MWCO endotoxin-low variant also available.
• Compatible rotors: swing-bucket and 25° fixed-angle; centrifugal force up to 3,000 x g (swing-bucket) or 6,000 x g (25° fixed-angle).

Applications

• Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
• Concentration of cerebrospinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
• Protein removal from serum and from cell or tissue lysates.
• Buffer exchange for proteomic peptide samples.
• Sample preparation for mass spectrometry.

Performance (time to concentrate up to 30x [min.] at 20°C and solute recovery %)
Aprotinin 0.25 mg/ml (6,500 MW): 5,000 MWCO HY — swing-bucket Min. 47 min, Recovery 95% ; 25° fixed-angle Min. 45 min, Recovery 95%

Cytochrome c 0.25 mg/ml (12,400 MW): 5,000 MWCO HY — swing-bucket Min. 45 min, Recovery 96% ; 10,000 MWCO HY — swing-bucket Min. 25 min, Recovery 94% ; 25° fixed-angle Min. 45 min, Recovery 96% ; 25° fixed-angle Min. 18 min, Recovery 94%

α-chymotrypsin 0.25 mg/ml (25,000 MW): 5,000 MWCO HY — swing-bucket Min. 50 min, Recovery 98% ; 10,000 MWCO HY — swing-bucket Min. 25 min, Recovery 98% ; 25° fixed-angle Min. 45 min, Recovery 98% ; 25° fixed-angle Min. 18 min, Recovery 98%

Ovalbumin 1.0 mg/ml (45,000 MW): 10,000 MWCO HY — swing-bucket Min. 20 min, Recovery 98% ; 30,000 MWCO HY — swing-bucket Min. 15 min, Recovery 94% ; 25° fixed-angle Min. 14 min, Recovery 98% ; 25° fixed-angle Min. 12 min, Recovery 94%

BSA 1.0 mg/ml (66,000 MW): 30,000 MWCO HY — swing-bucket Min. 18 min, Recovery 98% ; 25° fixed-angle Min. 15 min, Recovery 98%

IgG 0.25 mg/ml (160,000 MW): 30,000 MWCO HY — swing-bucket Min. 30 min, Recovery 98% ; 25° fixed-angle Min. 25 min, Recovery 96%

* Proteins other than IgG made up in 50 mM potassium phosphate, 150 mM sodium chloride, pH 7.4

Ordering options
Available with Hydrosart® membranes in MWCOs 2,000; 5,000; 10,000; 30,000 and pack sizes typically 12 or 48. A cellulose triacetate 20,000 MWCO endotoxin-low variant is offered as a separate option.

Technical specifications

• Concentrator capacity: swing-bucket rotor 15 ml; 25° fixed-angle rotor 12.5 ml.
• Dimensions: total length 116 mm; width 30 mm; active membrane area 3.9 cm².
• Hold-up volume (membrane): <20 µl.
• Dead stop volume: 30 µl.
• Materials: body — polycarbonate; filtrate vessel — polycarbonate; concentrator cap — polypropylene; membrane — Hydrosart® (modified regenerated cellulose).
• Centrifugal force: up to 3,000 x g (swing-bucket) or 6,000 x g (25° fixed-angle) depending on rotor.
• Recommended pipette: fixed or variable volume with thin gel-loader tips for concentrate recovery.