Stain reagent

staining solutionlaboratoryfor research

Product overview:
Acid‑fast stain (Ziehl‑Neelsen) BA4197 by Baso for automated stainers. Designed for detection of acid‑fast bacteria such as Mycobacterium tuberculosis using an improved basic fuchsin (Ziehl‑Neelsen) method. Supplied as a kit in 4×1000 ml format compatible with standard automated staining platforms.

Highlights:

• Compatible with automated stainers
• Improved basic fuchsin Ziehl‑Neelsen method for clear, rapid visualization
• Two counterstain options: methylene blue or malachite green

Testing principle:
Acid‑fast bacteria possess a lipid‑rich cell envelope that resists conventional staining. After staining with an enhanced dye (carbolfuchsin), the dye–lipid complex resists decolorization by acid‑alcohol, allowing acid‑fast organisms to retain the red stain and be differentiated from non‑acid‑fast microorganisms. This kit follows the Ziehl‑Neelsen (carbolfuchsin) principle recommended by WHO and uses an improved basic fuchsin method rather than fluorescent techniques.

Stain results:

• Methylene blue counterstain: acid‑fast bacteria appear red on a light‑blue background; other bacteria and cells appear blue.
• Malachite green counterstain: acid‑fast bacteria appear red on a light‑green background; other bacteria and cells appear green.
• Typical Mycobacterium tuberculosis morphology: bacilli, slightly curved, width ~0.3–0.6 µm, length typically 0.5–8 µm (most 1.5–3.5 µm); well‑stained organisms may show metachromatic granules and are observable under 100× oil immersion.

Packaging & specifications:

• Product Name: Acid Fast Stain (Ziehl‑Neelsen) (For Auto Stainer)
• Ref #: BA4197
• Specification: 4 x 1000 ml
• Place of Origin: China
• Brand Name: Baso
• Certification: IVDR Class A; ISO 13485:2016
• Applicable Departments: Hospital, Clinic, Research Institution
• Minimum Order Quantity (MOQ): 1 Box
• Standard Packaging: Paper box
• Delivery Period: To be confirmed
• Payment Method: T/T
• Supply Capacity: To be confirmed

Usage & storage:
For professional diagnostic laboratory use. Follow the automated stainer manufacturer's protocol and local laboratory biosafety procedures. Store reagents as indicated in the product instructions; avoid freezing and discard after expiry. Consult the package insert for detailed handling, compatibility and safety information.