Solution reagent EGSCG

cholesterolenzymatic

Serum Cholyglycine (Cholyglycine, CG) is one of the binding cholic acid combined by cholic acid and glycine, within the liver cells, cholesterol transformed into the primary bile acid through the extremely complex enzymatic reaction. Including cholic acid (CA) and chenodeoxycholic acid (CD - CA). there are three hydroxyl (C3, C7, C12) in the steroid nucleus of cholic acid, the hydroxyl at the end of side chains using peptide bonds with glycine, the molecular weight is 462u[1] Under normal circumstances, the cholic acid content in the peripheral blood is very little, no matter on an empty stomach or after eating, the concentration of serum CG is stable at a low level of a normal adult. When the liver cell is damaged, liver cells’ ability of absorbing CG will drop, cause the increase of the CG content in the serum; When the bile stasis, the liver’s excretion of bile acid occurs disorder, and the CG content in the reflux circulated blood is increase, also makes the blood CG content increased [1] Cholestasis syndrome during pregnancy (ICP), at present, the recognized index of ICP is CG values, which sensitivity is much higher than that of TBA, so at present’s clinic, the TBA cannot replace CG at all. Sensitivity For analysis sensitivity, the absorbance change rate (△A/min) should more than 0.0500 under sample concentration of 10mg/L. Interference Analytes Concentration Bilirubin 40 mg/dl Hemoglobin 500 mg/dl Intralipid 250 mg/dl RF 400 IU/ml Linearity Linearity is 2.5-80 mg/L