Sample is reacted with a buffer and anti-Cys C coated latex.The formation of the antibody-antigen complex during the reaction results in an increase in turbidity , the extent of which is measured as the amount of light absorbed at 570 nm.By constructing a standar curve from the absorbance of the standardsx Cystatin C concentration of sample can be determined.
ASSAY SPECIFICATIONS
Melhotl - Latex cnhanccd IT
Sample - Sérum, plasma trcateil wilh lithium heparinute
Calibrators - 5 vials:0.5.1.2.4.8 mg/1,; Liquid. rcady-to-use
Controls - l.iquid. ready-to-use
Assay time* - 10 minutes
Linearity - l lp tu 8.0 mg.'l.
Ratio - 1:1:3:1:5:1
Assay platform - Clinical chemisirv analyzers from Roche, Beckman. Abbott etc.
INTERFERENCE
The following analytes concentrations were not found to affect the assay:
1 Icmoglobin - up to 500 mg/dl
Dilirubin - up to 20 mg/dl
Intralipid - up to 2500 mg.'dl
RF - up to 500 mgdl