Dye reagent GS191Z

for biochemistrynon-esterified fatty acids

Non-esterified fatty acids (NEFA) and coenzyme A (CoA) react in the presence of Acyl-CoA synthetase (ACS) to acylated coenzyme A. Acylated coenzyme A is oxidized by acyl coenzyme A oxidase under development of H202. H202 is converted to a coloured product by the use of Trinder substances in the presence of peroxidase (POD). At 546nm the intensity of the dye is directly proportional to the concentration of free£ fatty acids in the sample. SPECIFICATIONS ACCURACY - The bias of control is ±10%. PRECISION - The CV of the test should be ≤5%. LINEARITY - The assay is linear up to 3 mmol/L. INTERFERENCE - The following analytes were tested up to the levels indicated and found not to interfEre: Intralipid: 300 mg/dL; Hemoglobin: 300 mg/dL Bilirubin: 40 mg/dL; Ascorbic acid: SO mg/dL