Sodium is determined enzymatically via sodium dépendent ft-galactosidase activity with ONPG as substrate. The absorbance at 405 nm of the product O-nitrophenyl is proportional to the sodium concentration.
SPECIFICATIONS
ACCURACY - The bias of control is ±10%.
PRECISION - The CV of the test should be <5%.
LINEARITY - The assay is linear up to 195 mmol/L.
INTERFERENCE - The following analytes were tested up to the levels indicated and found not to interfère: Intralipid: 1000 mg/dL; Hemoglobin: 500 mg/dL Bilirubin: 50 mg/dL; Ascorbic acid: 50 mg/dL