Research test kit KAPA

for DNA quantificationfor RNAfor DNA

Overview
KAPA Library Quantification Kits provide a complete solution for accurate, reliable and reproducible qPCR-based quantification of next-generation sequencing (NGS) libraries. Kits include KAPA SYBR FAST qPCR Master Mix (formulated with different passive reference dyes for different qPCR instruments), a sequencing platform-specific library quantification primer premix, and a pre-diluted set of DNA standards.

Contents

• KAPA SYBR FAST qPCR Master Mix (2X) — available in universal and instrument-specific reference-dye formulations (ROX High, ROX Low or instrument-specific dye)
• 10X library quantification primer premix (sequencing platform–specific)
• Pre-diluted set of DNA standards (Illumina DNA Standards 1–6)

Benefits at a glance

• Complete reagents for absolute qPCR-based quantification of individual NGS libraries or indexed library pools
• Standard curves support multiple library construction workflows, including PCR-free methods
• Quantification targets sequencing-competent library fragments only
• Pre-diluted standards with high lot-to-lot consistency simplify workflows and improve reproducibility
• Data analysis templates and sequencing support available to assist successful runs

Performance and reliability
KAPA SYBR FAST DNA polymerase is formulated to amplify diverse DNA fragments with comparable efficiency, enabling accurate quantification across heterogeneous library populations. Validation data show consistent amplification efficiencies across different amplicons and production lots, supporting reproducible cluster densities and consistent sequencing results.

Key applications

• Whole Genome Sequencing (WGS)
• Whole Exome Sequencing (WES)
• Targeted sequencing (custom panels)
• RNA-Seq
• ChIP-Seq
• Amplicon sequencing
• Methyl-Seq

Available kit variants and related products

• Lib Quant Kit (ABI) — complete kit (500 reactions)
• Lib Quant Kit (Uni) — complete kit (universal; 500 reactions)
• Lib Quant Kit (BioRad) — complete kit (500 reactions)
• Lib Quant Kit (ROX Low) — complete kit (500 reactions)
• Lib Quant Kit (LC480) — complete kit (500 reactions)
• LQ Stds 0 - 6 — DNA standards set (140 reactions)
• LQ Stds — DNA standards set (120 reactions)
• LQ Control Standard — control standard (20 reactions)
• LQ Primer + KSF (Uni) — primer + KAPA SYBR FAST (500 reactions)
• LQ Primer + KSF (LC480) — primer + KAPA SYBR FAST (500 reactions)

Notes on workflow impact
qPCR-based quantification measures sequencing-competent fragments and enables predictable cluster densities, effective equimolar pooling for multiplexed sequencing, and reduced need for lane titration. Kits are formulated to deliver consistent results with variable-quality samples, including FFPE-derived DNA.

Specifications

• Compatible platform: Illumina ecosystem
• Library type: any NGS library
• Starting material: any NGS library ready for cluster amplification
• Standard curve concentration range: 20–0.0002 pM (Illumina libraries)
• Sequencing applications: WGS, WES, targeted sequencing, RNA-Seq, ChIP-Seq, amplicon sequencing, Methyl-Seq
• Storage temperature: store at -15°C to -25°C