DNA ligase reagent KAPA EvoPlus Boost Kit

enzymeDNA polymerasefor DNA library preparation

Product overview
KAPA EvoPlus Boost Kits are DNA library preparation kits with integrated enzymatic fragmentation, designed to provide high-quality NGS libraries from a wide range of sample types and DNA inputs. Kits combine enzymatic fragmentation (KAPA EvoPlus FragTail chemistry) with optimized reagents and an amplification module (KAPA EvoAmp ReadyMix powered by HiFi) in workflows that are streamlined, automation-friendly, and suited for whole genome, whole exome and targeted sequencing.

Core benefits

• Highly streamlined, automation-friendly workflows that produce sequencing- or target capture-ready libraries in ~2 hours.
• Reduced hands-on time and fewer enzymatic steps with ready-to-use (ReadyMix) reagents and fewer pipetting steps.
• Automatable end-to-end protocol from sample to library, including tunable enzymatic fragmentation.
• Available as tube-based or plated reagent configurations to suit laboratory throughput and project size.
• Validated with KAPA UDI and KAPA HyperPlex adapters and with KAPA HyperPure Beads (sold separately) and compatible with KAPA target enrichment workflows and probes.
• Designed to minimize sequencing artifacts and deliver high fidelity, excellent coverage uniformity and efficient conversion of input DNA to library molecules.

Reliable, tunable fragmentation

• Adjust library insert size by varying fragmentation time (tunable insert size range ~250–1000 bp demonstrated).
• Reproducible insert sizes across a range of DNA inputs and GC contents.
• Robust performance with real-life samples, including DNA eluted in buffers containing EDTA (up to 2 mM) and other common inhibitors.

Performance and sequencing economy

• Engineered KAPA Evo T4 Ligase supports efficient conversion of input DNA to library molecules, improving yields from low inputs and difficult samples (both PCR-free WGS and amplified workflows).
• KAPA EvoAmp ReadyMix (powered by HiFi) provides low error rates and superior coverage uniformity, supporting high-confidence results and reduced sequencing requirements.
• Low-bias enzymatic fragmentation and optimized amplification minimize chimeras and other sequencing artifacts.

Sustainability and kit design

• Innovative formulations and kit configurations reduce the number of individual reagent containers and pipetting steps, lowering plastic waste and packaging.
• Kits are REACH compliant.

Typical applications

• Whole genome sequencing (WGS), including PCR-free WGS workflows.
• Whole exome sequencing (WES) when used with KAPA HyperCap workflows.
• Targeted sequencing, validated with KAPA HyperCap and KAPA HyperPETE target enrichment workflows and probes.

Product variants and format notes
Available variants include PCR-free formats and Boost (amplification) formats, in multiple pack sizes and plated or tube-based configurations. The Boost Kits include the KAPA EvoAmp ReadyMix powered by HiFi for applications requiring amplification; PCR-free variants are available for workflows where amplification is omitted.

Technical specifications

• Compatible platform: All Illumina sequencing systems
• Applications: WGS, WES, targeted sequencing
• Starting material: Genomic DNA, low-quality DNA (e.g., FFPE/FFPET DNA)
• Input amount: 0.1 ng – 500 ng (dependent on sample type, quality and amount; PCR-free from 50 ng)
• Storage conditions: Kits must be stored at -15°C to -20°C
• Fragmentation method: Integrated enzymatic fragmentation (tunable by time)
• Adapter compatibility: KAPA UDI Adapters and KAPA HyperPlex Adapters (KAPA adapters recommended)
• Typical workflow time: Sequencing-ready libraries in ~2 hours (varies with sample number, amplification step and automation)