Sample preparation reagent kit KAPA RNA EvoPrep

DNA polymeraseDNA ligasefor research

Overview
The KAPA RNA EvoPrep Kits provide a streamlined RNA library preparation workflow for next-generation sequencing (NGS). Building on the KAPA RNA HyperPrep Kit lineage, the EvoPrep Kits convert input RNA into adapter-ligated libraries ready for sequencing, with fewer steps and reduced hands-on time. The product is intended For Research Use Only (RUO) and not for diagnostic procedures.

KAPA RNA EvoPrep Kit for RNA library prep

• Fast, 3.5-hour workflow with minimal hands-on time
• Sensitive: detects more unique transcripts, including from low-input and degraded (FFPET) RNA
• Reliable: high concordance across input amounts and reproducible results

Key benefits and highlights

• Simplified workflow reduces hands-on time, reagent usage and plasticware
• Improved library conversion rates and higher yields across a wide range of inputs
• Increased capture of unique molecules (improved mapped unique rate, lower duplication and lower adapter-dimer percentages)
• Automation-friendly: compatible with liquid-handling automation and Roche AVENIO Edge System workflows
• Includes improved ligation chemistry (KAPA EvoT4 DNA ligase) and KAPA HiFi HotStart DNA Polymerase in amplification mix for low-bias, high-fidelity PCR

Performance and sensitivity
The kits show enhanced performance compared with previous KAPA RNA HyperPrep Kits and several competitor kits: detects more genes and transcripts across low-quality FFPET inputs and across low (5 ng) to higher inputs (up to recommended kit maxima). Figures reported on the page highlight: higher number of genes detected in low-quality FFPET samples, higher mapped unique rates, reduced duplication rates and lower adapter-dimer percentages.

Workflow summary

• Optional upfront enrichment: either mRNA capture (poly(A) oligo-dT beads) or rRNA and/or globin depletion (hybridization with complementary DNA oligos followed by RNase H/DNase treatment)
• RNA fragmentation (heat + magnesium)
• 1st strand cDNA synthesis by random priming
• Combined 2nd strand synthesis and A-tailing (dUTP incorporated into 2nd strand for strand specificity)
• Adapter ligation (TA-ligation to A-tailed fragments)
• Library amplification using high-fidelity polymerase (strand marked with dUTP is not amplified to enable strand specificity)

Compatibility and recommended inputs
Table (product variants and typical inputs):
Product variant — Typical reactions — EAN
KAPA RNA EvoPrep (24rxn) — 24 reactions — 07613336240564
KAPA RNA EvoPrep (96rxn) — 96 reactions — 07613336240557
KAPA RNA EvoPrep Kit (384rxn) — 384 reactions — 07613336239308
KAPA mRNA EvoPrep (24rxn) — 24 reactions — 07613336239377
KAPA mRNA EvoPrep (96rxn) — 96 reactions — 07613336239384
KAPA RNA EvoPrep+RiboErase HMR (24rxn) — 24 reactions — 07613336239315
KAPA RNA EvoPrep+RiboErase HMR (96rxn) — 96 reactions — 07613336239322
KAPA RNA EvoPrep+RiboErase HMR (384rxn) — 384 reactions — 07613336239339
KAPA RNA EvoPrep+RiboErase Globin (96rxn) — 96 reactions — 07613336239353
KAPA RNA EvoPrep+RiboErase Globin (384rxn) — 384 reactions — 07613336239360

Notes on inputs and sample types: recommended input ranges vary by kit variant (examples include ranges such as 0.25 ng–100 ng for certain RNA EvoPrep kits, 5 ng–1 µg or 25 ng–1 µg for others). KAPA mRNA EvoPrep is intended for high-quality RNA (RIN ≥ 7) and poly(A)-selected workflows; kits with RiboErase (HMR) / Globin are intended for human/mouse/rat depletion and are compatible with low-quality FFPE RNA when used with appropriate inputs and protocol adjustments.

Storage and handling
Typical storage guidance: temperature-sensitive components (cDNA synthesis and library prep reagents) should be stored at –15°C to –25°C; some upfront enrichment components (e.g., mRNA capture) stored at 2°C–8°C; KAPA HyperPure Beads stored at 2°C–8°C. Follow the Instructions for Use for exact storage and handling and recommended safe stopping points in the protocol.

Regulatory / usage note
For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Product family: KAPA RNA EvoPrep
• Primary application: RNA library preparation for NGS (whole transcriptome, gene expression, splice junctions, gene fusions, lncRNA)
• Enrichment options: no enrichment (total RNA), mRNA capture (poly(A)), rRNA and/or globin depletion (HMR/Globin)
• Input ranges (examples by kit): 0.25 ng–100 ng; 5 ng–1 µg; 25 ng–1 µg (depends on specific kit variant)
• Library prep time (typical): ~3.5 hours (streamlined protocol)
• Fragmentation: heat + magnesium, tunable for input quality
• Strand specificity: Yes (dUTP incorporation in 2nd strand; strand-specific sequencing)
• Ligation chemistry: KAPA EvoT4 DNA ligase
• Amplification polymerase: KAPA HiFi HotStart DNA Polymerase
• Bead cleanup: KAPA HyperPure Beads supplied for purification steps
• Automation: Compatible with liquid-handling automation and Roche AVENIO Edge System
• Species compatibility: KAPA RNA EvoPrep (no upfront enrichment) — broad (eukaryotic, microbe, plant, etc.); Kits with RiboErase (HMR)/Globin — human, mouse, rat depletion oligos
• Typical advantages: improved sensitivity (more unique transcripts), higher mapped-unique rates, lower duplication and adapter-dimer rates, fewer reagents/cleanups