DNA polymerase reagent KAPA EvoPrep Boost

reagent mediumenzymefor PCR

Summary
KAPA EvoPrep Boost Kits enable pre-fragmented DNA library preparation for WGS, WES, targeted sequencing and methyl-seq using a streamlined, automation-friendly workflow. Built on Roche's Evo chemistry, the Boost Kits combine the KAPA EvoPlus chemistry with the KAPA EvoAmp ReadyMix (powered by HiFi) to provide high fidelity, coverage uniformity and robust performance on fragmented DNA inputs including mechanically sheared DNA, cfDNA and FFPE DNA.

Key features and benefits

• One core chemistry for manual or automated preparation of libraries from varied DNA inputs and a wide range of applications.
• Streamlined workflow with fewer steps and reagents; ligation time reduced to ~5 minutes with KAPA Evo T4 ligase.
• ReadyMix formulations reduce pipetting steps and lower potential for error.
• Reagents available in tubes and plated formats for convenience and automation compatibility.
• Validated with KAPA UDI and KAPA HyperPlex adapters and with KAPA HyperPure Beads (sold separately).
• KAPA EvoPrep Boost Kit includes KAPA EvoAmp ReadyMix (powered by HiFi) for library amplification workflows; a PCR-free KAPA EvoPrep option is available for WGS from mechanically sheared DNA.

Benefits at a glance

• Flexible, streamlined workflows improve efficiency and save time.
• Reduced workflow time with fewer steps and reagents; only ~5 min ligation using KAPA Evo T4 ligase.
• Lower potential for error with ReadyMix formulations and fewer pipetting steps.
• Increased convenience and automation compatibility with tube and plate formats and accessory kits.
• High-efficiency target enrichment validated with KAPA HyperCap and KAPA HyperPETE workflows and KAPA target enrichment probes.

Performance highlights

• Improved coverage uniformity across GC bins for human and bacterial genomes, reducing the need for additional sequencing to cover underrepresented regions.
• Low error and artifact rates when using the Boost Kit together with KAPA EvoAmp ReadyMix powered by KAPA HiFi.
• High specificity and unique molecule recovery from cfDNA inputs in targeted sequencing workflows (demonstrated with KAPA HyperCap Oncology Panel on cfDNA).

Product variants

• KAPA EvoPrep Boost Kit (24 rxn)
• KAPA EvoPrep Boost Kit (96 rxn)
• KAPA EvoPrep Boost Kit, plated (96 rxn)
• KAPA EvoPrep Boost Kit (384 rxn)
• KAPA EvoPrep Boost PCR-free (24 rxn, 96 rxn, 384 rxn)

Product specifications
Compatible platform: All Illumina sequencing instruments
Application: WGS, WES, targeted sequencing, methyl-seq
Starting material: Fragmented DNA, cfDNA, FFPE DNA, PCR amplicons
Input amount: 0.1 ng - 500 ng*
Storage conditions: Store kits at -15°C to -20°C (readymixes are temperature sensitive)

Notes
*Input range depends on sample type, quality and workflow. PCR-free workflows supported from 50 ng and higher depending on kit variant.

Technical specifications

• Family: KAPA (Roche)
• Intended use: Research Use Only (not for diagnostic procedures)
• Compatible adapters: KAPA UDI Adapters and KAPA HyperPlex Adapters (validation stated)
• Included amplification reagent in Boost Kits: KAPA EvoAmp ReadyMix (powered by HiFi, 2X) for amplification-enabled workflows
• Formats: tube and plated kit configurations; plated full-length UDI adapters available in 96-well format
• Storage and handling: ReadyMix reagents are temperature sensitive; store at -15°C to -25°C on receipt; ship on dry ice or ice packs depending on destination
• Validated applications: whole genome sequencing (WGS), whole exome sequencing (WES), targeted sequencing, methyl-seq, and liquid biopsy cfDNA workflows