Solution reagent kit KAPA HyperPETE

for researchfor DNA

KAPA HyperPETE Workflow is a novel hybrid-capture technology designed to employ primer extension reactions to specifically capture and release target library molecules for sequencing. The KAPA HyperPETE Catalog Panels include readily available fixed-design panels for pan-cancer variants (with an MSI module), oncology hotspots, hereditary oncology, lung cancer fusion variants, and newborn screening. These fixed-design panels leverage over a decade of probe-design experience. Benefits at a glance Developed to uncover critical genomic information from difficult sample types Tissue RNA (FFPET) Achieves highly specific enrichment from a broad range of FFPET RNA input amounts (Figure 1) Detects all fusions present in reference cell line samples (Table 1) Plasma cfDNA Achieve high % reads on-target and high exon coverage (at ≥1000X) for a range of cfDNA input amounts (Figure 2) Detect variants with high sensitivity, from a range of plasma input cfDNA amounts Performance using FFPE RNA with the KAPA HyperPETE LC Fusion Panel. The KAPA HyperPETE Lung Cancer Fusion Panel achieved 92%-97% reads on-target from 10 ng and 50 ng of all FFPET samples tested. High specificity was demonstrated by the high percentage of reads on-target when starting from 10 ng or 50 ng of various quality (low, medium, high) input FFPE RNA. On-target rate was 92% to 97% (includes housekeeping and fusion targets) with good performance across all sample input amounts, qualities, and types. The on-target rate was calculated following rRNA read removal (0.8% to 11% of all reads). Two (2) cell-line samples (in duplicates) and fourteen (14) normal adjacent FFPET samples were used to assess performance.