Solution reagent kit KAPA HyperPlex

for research

For Research Use Only. Not for use in diagnostic procedures. Overview Truncated adapters are compatible with ligation-based library construction workflows for direct and targeted multiplexed DNA and RNA (cDNA) sequencing applications on the Illumina® platform. Sequencing barcodes are added with UDI primer mixes during the library amplification step. KAPA HyperPlex Adapters have a standard Illumina adapter backbone. They are therefore easily incorporated in new and existing Illumina sequencing workflows, and do not require customized sequencing primers. A combination of 8-nt sequencing barcodes on each of the i5 and i7 adapter oligos supports paired-end sequencing on 1- , 2- and 4-channel Illumina instruments. KAPA HyperPlex Adapters support a broad range of sample pooling (2- plex up to 384-plex, as appropriate for your workflow), for target enrichment and/or sequencing. Unique dual-indexing is recommended for all applications and Illumina sequencers. Product highlights Higher library complexity and fewer misassigned reads improve confidence in results. Unique dual-index combinations in KAPA HyperPlex Adapters allow reads with unexpected barcode combinations to be filtered out prior to data analysis. KAPA HyperPlex Adapters are manufactured using stringent procedures and undergo sequencing-based QC testing to reduce the potential for index misassignment resulting from barcode cross-contamination. KAPA Universal Adapter is offered with or without UMI (Unique Molecular Indexes) for germline or somatic variant detection, respectively KAPA HyperPlex Adapters deliver high library yields in applications that require library amplification