Solution reagent kit KAPA UDI

for researchliquid

Enrich accuracy and throughput with KAPA adapters and accessories For next-generation sequencing (NGS), using indexed adapters to barcode samples greatly increases efficiency. This process, known as multiplexing, allows many samples to be pooled in a single sequencing run, and the resulting reads are then sorted bioinformatically. However, some high-throughput sequencers are prone to index mis-assignment (index hopping), which can reduce data quality. Unique dual-indexing (UDI) strategies help to mitigate the impact of these events. Roche offers two types of KAPA adapters: Full-length adapters: For PCR-free sample prep with up to 96 index combinations KAPA UDI Adapters Benefits at a glance Low misassignment rates improve confidence in results Index misassignment during multiplexed sequencing can result from several factors, including: Index hopping Cross-contamination of barcodes or samples Template switching during PCR amplification of pooled samples Sequencing or analysis errors Some of these issues can be mitigated through careful adapter design and quality control. KAPA UDI Adapters are designed to support confident results through: Unique dual-index combinations that enable reads with unexpected barcode combinations to be filtered out prior to data analysis