NGS reagent KAPA

solutionreagent mediumfor hybridization

Overview
Efficient NGS target enrichment using Roche KAPA HyperCap and KAPA HyperPETE workflows. Solutions cover broad to focused panels and support custom designs and low‑input samples such as cfDNA.

Solution
Comprehensive target enrichment portfolio with two complementary technologies. The KAPA HyperCap Workflow is a hybrid capture–based method suited for larger panels and broad variant detection. The KAPA HyperPETE Workflow uses primer‑extension target enrichment (PETE) tailored for smaller, focused panels and ultra‑low inputs (e.g., cfDNA). KAPA HyperPETE combines amplicon‑like speed and workflow simplicity with hybrid‑capture performance including strong uniformity and on‑target rates.

Integrated offerings
Integrated workflows include high‑quality probes/primers and KAPA Library Prep Kits, supporting end‑to‑end sample-to-library processing for targeted sequencing projects.

• Exome probes: whole‑exome solutions optimized for comprehensive database coverage
• Catalog panels: fixed gene panels available from stock for rapid deployment
• Design Share panels: expert panels developed with research collaborators
• Custom probes: bespoke designs for any human or non‑human genomic regions

Key product types and highlights
Representative portfolio items and short descriptions:

• KAPA HyperExome V2 Probes and Kits: whole exome sequencing content designed for broad coverage
• AVENIO Edge System: fully automated IVD liquid handler for library preparation, target enrichment, quantification, normalization and pooling
• KAPA EvoPlus Boost Kits: DNA library preparation with enzymatic fragmentation and streamlined automation
• KAPA HyperCap Catalog Panels and Kits: gene panels for targeted enrichment research workflows
• KAPA HyperPETE Catalog Panels and Kits: fixed panels using KAPA HyperPETE hybrid‑capture technology
• Design Share Panels: NGS panel designs by Roche scientists and collaborators
• HyperDesign: custom design tool combining KAPA enrichment technology with Roche probe/primer expertise
• KAPA HyperCap Custom Probes / KAPA HyperPETE Custom Probes: custom target enrichment probes/panels for DNA and RNA regions

Value
Target enrichment concentrates sequencing reads on selected genomic regions, reducing cost and computational load versus whole‑genome approaches. This enables higher depth on targets, improving sensitivity for low‑frequency somatic variants in heterogeneous or low‑input samples—critical for oncology and translational research.

Vision
Roche provides catalog, pre‑designed and fully custom NGS target enrichment solutions to address sequencing challenges and deliver sensitive, precise results from low‑input or difficult targets.

Comparison / Considerations (summary)
A detailed comparison on the site contrasts KAPA HyperCap, KAPA HyperPETE and amplicon‑based workflows across ease of use, performance, technology, turnaround time, panel size, applications and analysis support. Key technical takeaways are summarized below.

Notes
Products and kits are intended for research use (RUO) where indicated.

Technical specifications

• Workflows: KAPA HyperCap Workflow (hybridization‑based capture); KAPA HyperPETE Workflow (Primer Extension Target Enrichment); Amplicon‑based workflows (PCR amplification)
• Ease of use: KAPA HyperCap – amenable to automation with reduced hands‑on time; KAPA HyperPETE – single‑day, automatable workflow; Amplicon‑based – fastest workflow
• Performance: KAPA HyperCap – high sequencing efficiency and detection across mutation classes; KAPA HyperPETE – high efficiency for small panels and detection across mutation classes; Amplicon‑based – good SNV/Indel detection, lower uniformity and limited CNV/novel fusion detection
• Turnaround time: KAPA HyperCap – ~1.5 days; KAPA HyperPETE – single day; Amplicon‑based – single shift
• Panel size: KAPA HyperCap – up to whole exome and beyond; KAPA HyperPETE – up to ~250 Kb; Amplicon‑based – up to a few megabases (practical limits apply)
• Applications: KAPA HyperCap – hereditary genetics and hereditary/somatic oncology research; KAPA HyperPETE – somatic oncology research and RNA fusion detection (known and novel partners); Amplicon‑based – somatic oncology and hereditary genetics (known fusions)
• Secondary analysis support: KAPA HyperCap & KAPA HyperPETE – germline and somatic secondary analysis supported with open‑source bioinformatic tools; amplicon‑based – vendor‑dependent