Infectious disease test kit

for animalsfor swineblood

Introduction
HWTAi® African Swine Fever Virus Real-time Fluorescent PCR Detection Kit (P72+CD2v+MGF+IC) uses real-time fluorescent PCR to amplify and detect P72, CD2v and MGF genes of ASFV. The kit is intended for detection of ASFV nucleic acid in whole blood, serum, plasma, lymph nodes, spleen, muscle, lung, oral fluid and environmental samples. An internal pig-derived DNA control (IC) is included to monitor sample quality and the amplification process. The quadruplex format supports routine surveillance, biosafety screening and differentiation between epidemic strains and strains with CD2v/MGF deletions.

Benefits

• One-step testing on whole blood, serum and plasma with minimal preprocessing.
• Simple workflow suitable for on-site use by trained veterinary or farm personnel.
• Rapid turnaround: results displayed in approximately 15 minutes; individual test time ≤ 10 seconds under high-throughput conditions.
• High throughput: up to 270 samples per hour at maximum speed.
• Compact and portable detection instrument with mobile terminal operation and cloud-based quality control.
• High analytical sensitivity comparable to enzyme-labeled assays; internal IC ensures validity of negative results.

Principle
The kit uses specific primers and fluorescent probes targeting the P72, CD2v and MGF genes of the ASFV genome in a quadruplex real-time PCR format. Fluorescent signal acquisition enables simultaneous detection of targets and the internal pig DNA reference, and allows discrimination between wild-type epidemic strains and deletion variants lacking CD2v and/or MGF sequences.

Components

• Test devices (reagents formatted for real-time PCR)
• Disposable droppers
• Single buffer
• Instructions for Use (IFU)

Related products
Related veterinary diagnostic kits: rapid test kits, ELISA kits and PCR/RT‑PCR kits for swine and ruminant pathogens (e.g. FMDV, BVDV, Brucella, common respiratory and enteric viruses).

INTERPRETATION OF ASSAY RESULT
Refer to the kit IFU for threshold values, internal control acceptance criteria and interpretation rules for target amplification signals. Follow laboratory quality management and local regulatory requirements for confirmatory testing.

Technical specifications

• Target genes: P72, CD2v, MGF; internal pig-derived DNA reference (IC).
• Assay type: Real-time fluorescent PCR, quadruplex.
• Sample types: whole blood, serum, plasma, lymph nodes, spleen, muscle, lung, oral fluid, environmental samples.
• Intended use: routine surveillance, biosafety screening, differential diagnosis of deletion strains.
• Result display time: approximately 15 minutes (documented); individual test cycle ≤ 10 seconds for high-throughput mode.
• Sensitivity: high analytical sensitivity, comparable to enzyme-labeled assays; use IFU for performance parameters.
• Package contents: test devices, droppers, single buffer, IFU.