Rapid infectious disease test

tuberculosisfor cattlegoat

Product overview
TB Test is a rapid immunochromatographic test kit for qualitative detection of anti-tuberculosis antibodies in cattle samples (whole blood, serum, plasma; milk noted). The assay provides a visual T line for antibody presence and a C control line to confirm test validity.

Introduction
This one-step lateral flow assay detects antibodies to Mycobacterium tuberculosis complex (including M. bovis and M. africanum) across immunoglobulin isotypes (IgG, IgM, IgA). It is designed for on-site screening and laboratory triage using minimally processed specimens.

Benefits

• Compatible with whole blood (fingerstick/venipuncture), serum and plasma; milk samples can be tested where applicable.
• Simple procedure suitable for non-specialist on-site inspection.
• Rapid time-to-result: interpretation in 5–10 minutes; typical display ~15 minutes.
• High throughput capability (manufacturer-reported up to 270 samples/hour under optimal conditions).
• Compact, portable format enabling mobile operation and cloud-based quality control integration.
• Reported sensitivity and specificity consistent with lateral flow enzyme-labeled comparators.

Principle
The assay uses a sandwich lateral flow immunochromatographic format with colloidal gold-labelled antigen. Anti-TB antibodies in the specimen bind labelled antigen and are captured at the test zone producing a visible T line; the C line must appear to validate the result.

Components

• Test devices (individual foil pouches)
• Droppers
• Single-use buffer
• Instruction for use (IFU)

Storage and stability
Store kit at 2–30°C (reported 4–30°C in some documentation). Do not freeze. Protect from direct sunlight. Shelf life: 18 months as marked on package label.

Test Procedure

• Collect fresh specimen (whole blood, serum or plasma). Test immediately after collection where possible.
• Remove test device from foil pouch and place horizontally.
• Add 2–3 drops (≈70–100 µL) of serum/plasma or appropriate volume of whole blood to the sample well; start timer.
• Read result between 5 and 10 minutes. Results read after 15 minutes are considered invalid.

Interpretation of assay result
If both C and T lines appear the specimen is positive for anti-TB antibodies. If only the C line appears the specimen is negative. Absence of the C line indicates an invalid test and repeat testing is required.

Table of specifications
Product Name: TB Test Bovine Tuberculosis Test Kit
Specimens: Whole blood (fingerstick/venipuncture), serum, plasma; milk specimen noted
Assay time: 5–10 minutes (interpretation); typical display ~15 minutes
MOQ: 800 test kits
Delivery time: 1 week after payment confirmation
Packing: 10 test kits/packing box
Storage temperature: 2–30°C (do not freeze); protect from direct sunlight
Shelf life: 18 months

Technical specifications

• Assay format: Sandwich lateral flow immunochromatographic assay (colloidal gold)
• Detected analyte: Anti-TB antibodies (IgG, IgM, IgA)
• Specimen volume: ~75 µL for serum/plasma; whole blood guidance ~70–100 µL
• Reading time: 5–10 minutes (results >15 minutes invalid)
• Reported performance: Relative sensitivity 83.8% (95% CI: 75.3%–90.3%), relative specificity 98.8% (95% CI: 97.2%–99.6%), relative accuracy 95.7% (95% CI: 93.6%–97.3%)
• Throughput: manufacturer claim up to 270 samples/hour at peak performance
• Contents: test devices, droppers, single buffer, IFU
• Packing: 10 tests per box; MOQ 800 kits