Gradient DNA amplification system LEIA-X series

real-timefor qPCRPeltier effect

Product Details
Brand: BIOBASE
Product origin: CHINA
Delivery time: Within 7 days
Supply capacity: Factory direct supply
Model: LEIA-X4; LEIA-X6

Introduction
Compact real-time qPCR system designed for clinical and research laboratories. Integrates assay design support, optimized qPCR reagents compatibility, intuitive data-analysis software and flexible hardware configurations to cover routine diagnostics, research and high-throughput verification workflows.

Applications

• Infectious disease testing and surveillance
• Food and water pathogen detection
• Pharmacology and drug analysis
• Stem cell and molecular biology research
• Oncology and genetic disease studies
• Plant science and agricultural biotechnology

Working Principle
Thermal cycling is driven by a semiconductor Peltier module for precise temperature steps. Fluorescence is measured by a high-sensitivity PD detector. Non-contact top excitation/detection with program-controlled channel switching and motorized X–Y scanning enables full-plate (96-well) readout. Final data processing and quantification are performed by dedicated software.

Features

• High-performance temperature control; module max ramp rate: 7.0℃/s.
• Top excitation/detection, non-contact measurement; no edge-effect optical correction required.
• High-sensitivity PD fluorescence detector and long-life LED excitation.
• Configurable 4‑channel (LEIA‑X4) or 6‑channel (LEIA‑X6) detection.
• User-friendly software with flexible protocol settings, comprehensive analysis and reporting.

Hardware
Durable thermoelectric cooling module (TEM) for reliable thermal cycling. Integrated scanning and heated-lid modules use compression springs, rubber sealing and cam/guide mechanisms to protect samples, reduce external light interference and ensure scanning accuracy.

Software
Functions include absolute quantification, relative quantification (ΔΔCt/AACT), melting curve analysis, SNP/genotyping, guided workflows, experiment templates and parameter storage for routine laboratory use.

Technical specifications

• Model: LEIA‑X4; LEIA‑X6
• Sample capacity: 96×0.1 ml plate, 12×8-strip tubes, 96×0.1 ml single tubes (transparent cover)
• Reaction volume: 5–50 μl
• Dynamic range: 1–1×10^10 copies
• Channels: LEIA‑X4 = 4; LEIA‑X6 = 6
• Excitation: LED; Detector: PD
• Excitation wavelength: 455–650 nm (LEIA‑X4); 455–685 nm (LEIA‑X6)
• Detection wavelength: 510–710 nm (LEIA‑X4); 510–740 nm (LEIA‑X6)
• Fluorescence repeatability: CV ≤ 3%; accuracy: CV ≤ 5%; linearity r ≥ 0.995
• Module temperature range: 4–105℃; ramp rate: up to 7.0℃/s
• Temperature accuracy: ±0.5℃; uniformity: ≤ ±1℃
• Gradient range: settable 30–105℃ (span 1–36℃)
• Hot-lid temperature: 100℃ (automatic)
• Scanning mode: top scanning; scanning time: 8.5 s (full plate)
• Operation system: Microsoft Windows 10; Port: USB
• Power supply: AC100–240V, 50/60Hz
• Dimensions (W×D×H): 375×505×375 mm; Net weight: 28 kg; Packing size: 645×565×605 mm; Gross weight: 49 kg