Solution reagent kit KB03004
for protein analysisproteincopper

solution reagent kit
solution reagent kit
solution reagent kit
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Characteristics

Type
solution
Applications
for protein analysis
Tested parameter
protein, copper
Storage temperature

4 °C
(39 °F)

Description

Lowry Protein Quantification Assay is based on Lowry method, first described in 1951. The method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds, forming reduced copper in alkaline solutions. This is called a Biuret chromophore and is commonly stabilized by the addition of tartrate. The second reaction is the reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate), primarily by the reduced copper-amide bond complex as well as by tyrosine, tryptophan, histidine, cysteine, and cysteine residues in the protein. The monovalent copper ion catalyzes the latter reaction. The reduced Folin-Ciocalteu reagent is blue and thus detectable with a spectrophotometer in the range of 500 to 750 nm. The Biuret reaction itself is not very sensitive. Using the Folin-Ciocalteu reagent to detect reduced copper makes the Lowry assay nearly 100 times more sensitive than the Biuret reaction alone. Additional information Sizes: 500 ml Expiry date: 1 year Assay time: 60 minutes Reagents: Reagent A, Reagent B, Reagent C, and Protein Standard

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ANALYTICA 2024
ANALYTICA 2024

9-12 Apr 2024 München (Germany) Hall A3.216 - Stand Vide

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