Automated flash chromatography system Isolera™ LS

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Overview
Automated flash chromatography system for process development and production scale-up. Isolera™ LS handles up to 150 g per run and integrates advanced gradient and detection technologies to support gram‑to‑hundred‑gram purification workflows.

Key benefits

• Scale-up from gram scale to multi‑ or hundred‑gram production with a compact laboratory footprint.
• Up to 30% solvent savings through Isolera Spektra Gradient Optimization.
• Quatro‑binary gradient enables use of up to four solvents in a single run to separate compounds with diverse polarity.
• Improved fraction and compound purity via PDA scanning, λ‑All spectral capture and baseline rise correction.

Software and features

• Isolera Spektra software provides gradient optimization and can construct gradients to separate up to six compounds in a single sample.
• Cartridge selection guidance based on loading capacity and purification speed; step‑gradient mode isolates target compounds while reducing run time and solvent use.
• PDA scanning and λ‑All measure individual UV spectra of eluting compounds for accurate detection and fraction tracking.

Applications

• Process development laboratories and production scale‑up operations.
• Designed to purify samples up to 150 g per run.

Technical specifications

• Dimensions (expanded bed): 565 mm × 596 mm × 497 mm (22” × 23.5” × 19.6”).
• Weight: approx. 40 kg depending on configuration.
• Flow rate range: 50–500 mL/min.
• Fraction collection modes: volume, threshold, threshold with volume, low slope, medium slope.
• Solvent delivery: two constant‑volume (11 mL) electric HPFC pumps.
• Power requirements: 100–240 VAC, 50/60 Hz, 4.0 A.
• Collection vessels: test tubes (13, 16, 18, 25 mm) and bottles (120 mL, 240 mL).
• UV detection: selectable variable‑wavelength (200–400 nm) or UV‑VIS (200–800 nm).
• UV collection modes: single / dual / λ‑All (variable UV and UV‑VIS).