OverviewBiotage® PhyPrep automates plasmid purification from cell pellets across Maxi, Mega and Giga scales, delivering transfection-grade, low-endotoxin plasmid DNA suitable for transfection, gene therapy research and vaccine development. The system supports scalable, hands-free workflows with consistent high-purity yields.
Key benefits- Automates Maxi, Mega and Giga plasmid purifications, reducing manual prep time by up to 40%.
- Delivers reproducible results with proprietary dual-flow chromatography for consistent batch-to-batch performance.
- User-friendly touchscreen interface, pre-aliquoted buffers and intuitive protocols for minimal setup.
- Hands-free operation, scalable from a few samples to production-scale runs.
Ordering information- 416700 — Biotage® PhyPrep Maxi Mega Giga (requires vacuum pump ME1C, part # 356330SP and vacuum pump accessory kit, part # 417550SP) — Pack size: 1 — Price: Get a quote
- SER-PHY-FYMP — First Year Maintenance Package - Phyprep — Pack size: 1 — Price: Get a quote
- SER-PHY-IN — Installation and Familiarization - Phyprep — Pack size: 1 — Price: Get a quote
- SER-PHY-SUP — Phyprep Startup Package — Pack size: 1 — Price: Get a quote
Technical specifications- Plasmid yield: Up to 1 / 5 / 10 mg from 2 / 4.5 / 7 g cell wet weight (Maxi / Mega / Giga) using rich media and a high-copy plasmid.
- Plasmid quality: Transfection-grade with ~80–90% supercoiled plasmid DNA (with rich media and high-copy plasmids).
- Throughput: 1–4 samples per run.
- Processing time: ~42 min (Maxi, 1–2 samples); ~298 min (Giga, 1–2 samples).
- Maximum yield per run: Up to 10 mg of high-purity, low endotoxin plasmid DNA.
- Chromatography: Proprietary dual-flow chromatography for consistent purity and low endotoxin levels.
- Software/interface: Intuitive touchscreen interface, minimal setup and pre-aliquoted buffers to streamline workflows.