Flash chromatography system Flash 400

for productionfor biopharmaceutical applicationsfor research and development

Overview
Biotage® Flash 400 is a skid-mounted flash chromatography system engineered for kilogram-scale purification and production environments. It provides a complete purification skid for scale-up from development to cGMP manufacturing and accommodates a wide range of stationary phases and solvent systems.

Benefits

• Enables faster scale-up: operates safely up to 100 psi (6.9 bar) to support higher flow rates and higher-viscosity solvents. Radial compression technology minimizes wall effects and channeling, maintaining bed stability for cleaner fractions and improved overall yield.
• Designed for cGMP production: supplied with an ASME "UM" stamp, CE and ATEX certifications, and certified for use in Japan, Europe and North America. Each system is delivered with comprehensive engineering documentation, a certificate of performance and a certificate of compliance to support validation filing.
• Safety for high-solvent operations: configurable for explosion-proof, non-sparking areas. Air-driven pump and hoists, proper grounding and pressure-relief devices are included.
• Flexible cartridge support: compatible with routine normal-phase flash and challenging separations using reversed-phase silica, activated carbon, low-metal/acid-washed silica, Mitsubishi Diaion™ HP20SS resins, ion-exchange media or custom/client-packed cartridges.

Resources (selected)

• Brochure: Scalable flash purification - From development to production
• Selection guide: Scale-up and method development resources
• Customer case studies: CordenPharma adoption; collaboration with Croda to support lipid supply for COVID-19 and mRNA vaccine development

Specifications

• Flow rate range (pressure controlled): up to 6 L/min; 2–5 L/min recommended
• Certifications: CE, ATEX, ASME
• Radial compression pressure: 80–100 psig (552–689 kPa)
• Weight: ~455 kg (1000 lbs), depending on configuration
• Dimensions (L×W×H): hoist retracted: 115 cm × 78 cm × 175 cm; hoist extended: 115 cm × 78 cm × 241 cm
• Inlet pressure range: 100 psi (6.9 bar)
• Solvent delivery: pneumatic solvent pump, up to 100 psig (6.9 bar)
• Power requirement: inert gas-driven system (no electrical power required); fully grounded
• Installation floor load requirement: 1,220 kg/m² (250 lbs/sq. ft.)