Antibody PA0416-U

monoclonal antibodyfor histopathologyimmunohistochemistry

Antigen Background
Basic calponin (calponin‑h1) is a 34 kD protein that binds actin, tropomyosin and calmodulin and is involved in regulation of smooth muscle contraction. Expression is largely restricted to smooth muscle cells and marks the differentiated contractile phenotype. Loss of basic calponin is associated with phenotypic switching of vascular smooth muscle cells to a synthetic state implicated in atherosclerosis and restenosis after angioplasty. Basic calponin may influence proliferation, transformed phenotype and metastatic potential via the cortical actin cytoskeleton. Basic calponin mRNA is detected in smooth muscle of prostate, bowel and aorta; neutral and acidic calponin isoforms are more widely expressed in non‑smooth muscle tissues.

Intended use / Disclaimer
Recommended for detection of Calponin (Basic) in normal and neoplastic tissues by immunohistochemistry as an adjunct to routine histopathology. For professional in vitro diagnostic use only. Laboratories must validate performance and follow local regulatory requirements before clinical use.

Characteristics / technical specifications

• Clone: 26A11
• Pathology panel: Muscle Pathology
• Configuration: BOND RTU (ready‑to‑use)
• HD (High Definition): No
• Use: P (HIER)
• Source: Mouse monoclonal
• Regulatory status: In Vitro Diagnostic Use
• Volume: 7 ml
• Available SKUs: PA0416‑U (7 ml Calponin B Bond RTU Primary), PA0416 (7 ml Calponin B Bond RTU Primary)
• Control tissues: prostate, bowel, aorta
• Storage: 2–8°C, do not freeze
• Interpretation: cytoplasmic staining in smooth muscle cells and basal cells (e.g. prostate); validate on expected tissues