Monoclonal antibody B-DG-CE

diagnosticimmunohistochemistryfor immunodetection

Product overview
Monoclonal antibody for detection of Beta-Dystroglycan (DAG1) in human tissues. Clone 43DAG1/8D5. Supplied as a lyophilised concentrate; nominal volume 1 ml after reconstitution. Intended for use in immunohistochemistry (IHC) on frozen sections and for applications in muscle pathology research and diagnostics.

Antigen background
Dystrophin-associated glycoproteins (DAGs) are involved in the attachment of dystrophin to muscle membranes. The biological significance of this dystrophin/glycoprotein complex is not fully understood, but it appears to form an essential linkage between actin on the inside of the muscle fiber and muscle laminin in the basal lamina which surrounds the fiber. Beta-dystroglycan spans the sarcolemma and it has been suggested that it is the member of the complex which binds directly to dystrophin.

Applications and image
Immunohistochemical staining has been demonstrated on frozen longitudinal sections of human skeletal muscle. Staining localizes to the sarcolemma of muscle fibres when tested with clone 43DAG1/8D5. Use appropriate positive and negative controls and membrane integrity markers where indicated.

Available product

• SKU: B-DG-CE — 1ml NCL-b-DG

Technical specifications

• Clone: 43DAG1/8D5
• Pathology menu: Muscle Pathology
• Configuration: Lyophilised Concentrate
• HD (High Definition): No
• Use: F
• Source: Mono
• Species: Mouse
• Regulatory status: In vitro diagnostic use
• Volume: 1ml

Disclaimer
Beta-Dystroglycan is recommended for the detection of specific antigens of interest in normal and abnormal tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains.