Veterinary test kit virotype BVDV RT-PCR Kit

for bovine viral diarrheafor cattleblood

Product Overview
Bovine Viral Diarrhea is an economically important infectious disease of cattle. The virotype BVDV RT-PCR Kit is designed for rapid and highly reliable identification of RNA from Bovine Viral Diarrhea Virus genotypes 1 and 2 (BVDV-1, BVDV-2) and atypical pestivirus strains (HoBi-like, BVDV-3) in cattle. This kit is intended for use outside the U.S. and Canada.

Intended Use
The virotype BVDV RT-PCR Kit is intended for the detection of BVDV RNA in blood, plasma, serum, milk, and ear tissue samples (individual or pooled) from cattle. It contains all the reagents required for real-time RT-PCR, including a positive and negative control. Both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination. An internal control is included to exclude the possibility of false negative results.

Key Features

• Two specific primer/probe combinations: one for pestiviruses (FAM™ fluorescence) and one for the internal control (HEX™ fluorescence).
• High sensitivity allows for pooled testing: blood samples (up to 50), ear tissue samples (up to 25), and milk samples (up to 100).
• Approved in Germany (since 2007), Switzerland (2018), France (2013), Czech Republic (2014), and Belgium (2014).
• Widely used in BVDV eradication and control programs (e.g., Ireland, Germany, UK).

Regulatory and Licensing Information
For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country; product may not be available in your geographic area. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada.

Product Contents
PCR Mix (includes primers, probes and internal control), Enzyme Mix, Positive Control, Negative Control, Handbook

Specifications

• Pathogen type: Virus
• Product type: Ready-to-use assay
• Species: Ruminants
• Pathogen investigated: Bovine Viral Diarrhea Virus (BVDV)
• Technology: RT-qPCR

References
1. Maes R, Bronson-Doherty P, Chumbley M, Herrmann DC, Wise A, Kull-Mason S, Miller V, Schroeder C (2017): Detection of Bovine Viral Diarrhea (BVD) Virus in Ear Skin Tissue Samples: Evaluation of a Combination of QIAGEN MagAttract 96 cador Pathogen Extraction and virotype BVDV RT-PCR-based Amplification, 16th Annual Meeting of the Canadian Animal Health Laboratorians Network (CAHLN), June 4-7, 2017, University of Guelph, Guelph, ON, Canada
2. Bauermann FV, Falkenberg SM, Vander Ley B, Decaro N, Brodersen BW, Harmon A, Hessman B, Flores EF, Ridpath JF (2014): Generation of Calves Persistently Infected with HoBi-Like Pestivirus and Comparison of Methods for Detection of These Persistent Infections, Journal of Clinical Microbiology, Volume 52 No. 11 p. 3845–3852
3. Hoffman B, Depner K, Schirrmeier, H, and Beer, M (2006): A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. of Virological Methods, Volume 136, Issues 1–2, pp.200–209.
4. Fux, R., and Wolf, G. (2012): Transient elimination of circulating bovine viral diarrhoea virus by colostral antibodies in persistently infected calves: a pitfall for BVDV-eradication programs? Veterinary Microbiology 161 13–19.

Characteristics / Technical Specifications

• Product contents: PCR Mix (includes primers, probes and internal control), Enzyme Mix, Positive Control, Negative Control, Handbook
• Pathogen type: Virus
• Product type: Ready-to-use assay
• Species: Ruminants
• Pathogen investigated: Bovine Viral Diarrhea Virus (BVDV)
• Technology: RT-qPCR