Veterinary assay kit virotype PRRSV EU Vax RT-PCR Kit

for respiratory diseasesfor swinevirus

Product Overview
The virotype PRRSV EU Vax RT-PCR Kit from INDICAL is the first assay to simultaneously detect and differentiate all major live-attenuated PRRSV-1 (EU genotype) vaccines. PRRSV-1 predominates in Europe but also occurs in Asia and America. This kit is designed for the simultaneous detection and differentiation of RNA from all major PRRSV-1 (EU) vaccines in one run.

Save Time and Reduce Costs

• Detect all major PRRSV-1 vaccines in a single run
• Rapidly and cost-effectively differentiate vaccines compared to sequencing
• Distinguish infected from vaccinated animals (DIVA) when combined with the virotype PRRSV 2.0 RT-PCR Kit (VT282325)

High Performance and Ease of Use

• Kit combines two ready-to-use Triplex RT-qPCRs covering all major PRRSV-1 vaccines
• Exogenous Internal Controls to monitor PCR efficiency
• Highly sensitive — detects as few as 5 copies per sample
• Results in just 67 minutes*
• Compatible with most PCR thermocyclers
• Optimized for IndiMag/IndiSpin extraction workflows

Kit Components
Figure 1: The PRRSV EU Vax RT-PCR Kit contains two ready to use Master Mixes, A and B, with the corresponding targets and detection channels detailed below (see product images for tables).

Flexible with Multiple Validated Swine Sample Types

• Serum
• Plasma
• Saliva
• Tissue
• Processing fluids

Background
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infections in swine are highly prevalent and economically very important for the swine industry. PRRSV is an RNA virus that belongs to the order Nidovirales, family Arteriviridae. PRRS virus infection can cause respiratory disease in piglets and reproductive failure in pregnant sows.
Monitoring vaccination success requires differentiating vaccine from wild-type PRRSV strains. Sequencing can be costly, time-consuming and incompatible with weak positive samples. The virotype PRRSV EU Vax RT-PCR Kit offers a fast, practical alternative to confirm PRRSV-1 vaccine status, saving time in the lab while delivering reliable, actionable results for confident herd health decisions.
Currently, four commercial live-attenuated vaccines are used for PRRSV-1 control (see product images for details).

Regulatory and Availability Notes
For up-to-date licensing information and product-specific disclaimers, see the respective handbook or user manual. Assays for veterinary use only. Regulatory requirements vary by country, product may not be available in your geographic area. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada.

Characteristics / Technical Specifications

• Pathogen type: Virus
• Product type: Ready-to-use assay
• Species: Swine
• Pathogen investigated: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
• Technology: RT-qPCR
• Cat. No.: VT282365