Product OverviewThe virotype SBV 2.0 RT-PCR Kit (FLI-C 115) from INDICAL enables rapid, safe, and sensitive detection of RNA from Schmallenberg Virus (SBV) in samples from ruminants. This kit is designed for fast, safe, and sensitive detection of RNA from Schmallenberg virus.
- Faster time to result: Ultra-fast protocol with a total RT-qPCR run time of approximately 1 hour*.
- Internal control for greater confidence: Endogenous Internal Control (β-actin) included.
- Ease of use: Ready-to-use reagents including a single reaction mix, saving precious lab time.
- Multiple validated sample types: Suitable for tissue samples, serum, blood (individual or pooled), semen, and (swabs of) meconium from ruminants.
- Validated pooling options: High sensitivity allows blood and serum samples to be tested in pools of up to 5 individual samples.**
- New virotype 2.0 family protocol for more efficient testing: Shares one cycling protocol, allowing the virotype SBV 2.0 RT-PCR Kit to be performed in one run with other virotype 2.0 RT-PCR Kits from INDICAL.
Schmallenberg Virus (SBV)SBV is an enveloped, single-stranded RNA virus belonging to the Bunyaviridae family, genus Orthobunyavirus. Transmission occurs mainly by biting midges (Culicoides spp.) and/or mosquitoes. Direct animal-to-animal transmission is unlikely, but vertical transmission from dam to fetus via the intrauterine route does occur. In adult cattle, clinical symptoms include a short period of reduced milk yield, diarrhea, and high fever. In sheep, weakness and diarrhea are the main clinical signs. Depending on the gestation period, infection with SBV can cause embryonic loss, mummification, abortion, stillbirth, or congenital malformations such as arthrogryposis-hydranencephaly syndrome (AHS).
*Depending on PCR-Cycler. See Handbook for detailed information.
**Pool recommendations and regulations may vary between countries.
Characteristics / Technical Specifications- Pathogen type: Virus
- Product type: Ready-to-use assay
- Species: Ruminants
- Pathogen investigated: Schmallenberg Virus (SBV)
- Technology: RT-qPCR
- Cat. No.: VT281625
- Kit size: 100 reactions
- Availability/distribution: Outside the U.S. and Canada