Product OverviewThe ANAPLASMA-ELISA DOG is an indirect enzyme immunoassay designed for the detection of IgG antibodies to Anaplasma phagocytophilum in serum and plasma samples from dogs. This assay is intended for veterinary use only and is available for distribution outside the U.S. and Canada.
Disease BackgroundAnaplasma phagocytophilum is the causative agent of anaplasmosis, a common tick-borne disease in dogs. Antibodies to Anaplasma phagocytophilum are generally detectable 10 to 14 days post-infection (2 to 5 days after morulae are detectable in stained blood smears). A significant rise in antibodies within 7 to 21 days is characteristic of an active Anaplasma infection. However, a high-level antibody titre persisting for several months does not indicate an acute infection. In cases of suspected anaplasmosis, it is recommended to monitor the dynamic titre alongside clinical symptoms.
Features and Benefits- Faster and easier to use compared to Indirect Immunofluorescence Antibody Tests (IFATs) for anaplasmosis
- High sensitivity of 92.5% and high specificity of up to 97% (validated with n = 154 characterized dog sera)
Workflow and Usability- Ready-to-use reagents (Positive/Negative Controls, Conjugate, Substrate, Stop Solution)
- Color-coded caps
- Interchangeable reagents between AFOSA assays
- Microplate with 12 individual strips of 8 wells
- All AFOSA ELISA kits use the same short protocol
- Unified pipetting volume of 100 µl
- Suitable for automated ELISA instruments
Regulatory and Availability NotesFor up-to-date licensing information and product-specific disclaimers, refer to the respective handbook or user manual. Regulatory requirements vary by country; product may not be available in all geographic areas. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada.
Product picture: www.hausdorf-art.com
Characteristics / Technical Specifications- Pathogen type: Bacteria
- Product contents: 1 Microtiter plate with 96 wells (coated with Anaplasma antigen), Sample dilution buffer, Wash buffer (10x concentrate), Conjugate solution, Substrate solution, Stop solution, Positive control serum, Negative control serum
- Product type: Ready-to-use assay
- Species: Dog
- Pathogen investigated: Anaplasma phagocytophilum (Anaplasmosis)
- Technology: ELISA