Veterinary assay kit virotype BTV pan/3 2.0 RT-PCR Kit

fertilityfor cattlefor sheep

Product Overview
The virotype BTV pan/3 2.0 RT-PCR Kit (FLI-C 158) from INDICAL enables rapid and reliable detection of Bluetongue Virus (BTV) RNA, and differentiation of European serotype BTV-3 specifically, in samples from cattle, sheep, and goats. This kit is designed for use outside the U.S. and Canada.

Key Features

• Enhanced, ultra-fast protocol with a total RT-qPCR runtime of approximately 1 hour*
• Can be performed in one cycler run with other virotype 2.0 assays, including virotype 2.0 BTV/pan 8 2.0 RT-PCR Kit
• Easy-to-use single reaction mix with endogenous internal extraction and amplification control
• Ready-to-use positive and negative controls

Performance

• Triplex RT-qPCR for identification of all known BTV serotypes plus separate detection of BTV-3 in one single PCR run
• Highly sensitive detection of as few as 5 copies per sample
• Usable on most common PCR thermocyclers
• High efficiency on all instruments tested

Sample Flexibility

• Ruminant whole blood (preferred with anticoagulants, e.g., EDTA-blood)
• Tissue samples (spleen, lymph nodes) from cattle, sheep, and goats

Due to the high sensitivity of the test, pools of up to 10 individual blood samples may be analyzed. However, the optimum pool size depends on the regional prevalence for BTV.**

Supply Certainty & Support

• Trusted supplier of high-quality qPCR test systems since 2007
• High production capacity and safety stocks
• Excellent technical support

Bluetongue and Bluetongue Virus (BTV)
Bluetongue is an infectious disease of ruminants caused by the Bluetongue Virus (BTV), a double-stranded RNA virus of the genus Orbivirus, family Reoviridae. BTV is widely distributed globally and primarily affects sheep, cattle, and goats. Camelids (such as alpacas) and wild ruminants (including deer and stags) can also be affected. The virus is mainly transmitted by mosquitoes of the genus Culicoides (midges), though transmission through contaminated needles is also possible.
Symptoms of Bluetongue include skin changes in the mouth area, a decline in milk yield, reduced general condition, abortions, fertility problems, and increased mortality in young animals. The characteristic blue coloration of the tongue is rarely observed.

*Depending on PCR-Cycler. See Handbook for detailed information.
**Pool recommendations and regulations may vary between countries.

Characteristics / Technical Specifications

• Product contents: Master Mix, Positive Control, Negative Control, Handbook
• Pathogen type: Virus
• Product type: Ready-to-use assay
• Species: Ruminants
• Pathogen investigated: Bluetongue Virus (BTV)
• Technology: RT-qPCR
• Cat. No.: VT280475