Veterinary assay kit LEISHMANIA-IgG ELISA DOG

leishmaniasisfor dogsIgG

Product Overview
The LEISHMANIA IgG ELISA DOG is an Enzyme Immunoassay designed for the detection of IgG antibodies to Leishmania infantum in serum and plasma samples from dogs. This assay is intended for veterinary use only and is distributed outside the U.S. and Canada.

Background
Leishmania infantum is the causative agent of leishmaniasis, a significant zoonosis occurring particularly in the tropics and sub-tropics of Africa, Asia, the Americas, and southern Europe. Dogs are the main reservoir host, and the disease can be fatal in both dogs and humans. Transmission occurs via the bite of infected sandflies, and dogs of all ages can be infected, with an incubation period ranging from months to years.

The disease may present symptomatically (with polyclonal B-cell activation, specific antibody generation, and cellular immunosuppression), subclinically (with a specific humoral immune response), or latently (with weak or absent humoral and cellular immune responses). Clinical symptoms, in decreasing frequency, include lymphadenopathy, skin reactions, cachexia, hyperthermia, conjunctivitis, splenomegaly, and abnormal claws. Laboratory findings often show slight to moderate normochromic anemia, moderate leukopenia, hypergammaglobulinemia, and hypoalbuminemia.

Features and Benefits

• Reliable test for detection of leishmaniasis in dogs
• High sensitivity up to 88.4% and high specificity up to 99.5% (validated with n = 50 characterized dog sera)

Workflow and Usability

• Ready-to-use reagents, including Positive/Negative Controls, Conjugate, Substrate, and Stop Solution
• Color-coded caps for easy identification
• Interchangeable reagents between AFOSA assays
• Microplate with 12 individual strips of 8 wells
• Suitable for automated ELISA instruments

Regulatory and Availability Notes
For up-to-date licensing information and product-specific disclaimers, refer to the respective handbook or user manual. Assays are for veterinary use only. Regulatory requirements vary by country; product may not be available in all geographic areas. PCR and ELISA kits availability/distribution: Outside the U.S. and Canada.

Product Contents
1 Microtitre plate (96 wells coated with antigen), Sample dilution buffer, Wash buffer (10x concentrate), Conjugate solution, Substrate solution, Stop solution, Positive control serum, Negative control serum

Characteristics / Technical Specifications

• Pathogen type: Parasite
• Product type: Ready-to-use assay
• Species: Dog
• Pathogen investigated: Leishmania infantum
• Technology: ELISA
• Cat. No.: LED-KIT (1)