Veterinary assay kit virotype BTV pan/8 2.0 RT-PCR Kit

for cattlefor sheepgoat

Product Overview
The virotype BTV pan/8 2.0 RT-PCR Kit (FLI-C 114) from INDICAL enables rapid and reliable detection of Bluetongue Virus (BTV) RNA, and differentiation of European serotype BTV-8 specifically, in samples from cattle, sheep, and goats. This kit is designed for the detection of Bluetongue Virus RNA plus separate identification of the European serotype BTV-8. Availability/distribution: Outside the U.S. and Canada.

Key Features

• Enhanced, ultra-fast protocol with a total RT-qPCR runtime of approximately 1 hour*
• Can be performed in one cycler run with other virotype 2.0 assays
• Easy-to-use single reaction mix with endogenous internal extraction and amplification control
• Ready-to-use positive and negative controls

Performance

• Triplex RT-qPCR for identification of all known 36 BTV serotypes plus separate detection of BTV-8 in one single PCR run
• Highly sensitive detection of as few as 5 copies per sample
• Usable on most common PCR thermocyclers
• High efficiency on all instruments tested

Sample Flexibility

• Ruminant whole blood (preferred with anticoagulants, e.g., EDTA-blood)
• Tissue samples (spleen, lymph nodes) from cattle, sheep, and goats

Pools of up to 5 individual blood samples may be analyzed due to the high sensitivity of the test. The optimum pool size depends on the regional prevalence for BTV.**

Supply Certainty & Support

• Trusted supplier of high-quality qPCR test systems since 2007
• High production capacity and safety stocks
• Excellent technical support by veterinarians

Bluetongue Disease and Bluetongue Virus (BTV)
Bluetongue is an infectious, non-contagious disease of ruminants caused by Bluetongue Virus (BTV), a double-stranded RNA virus of the genus Orbivirus (family Reoviridae), which includes 36 known serotypes including atypical BTV. BTV is widely distributed around the world, mainly affecting sheep, cattle, and goats. Clear clinical signs are usually seen only in sheep, with severe cases showing intense hyperemia and cyanosis of the tongue (Bluetongue).
BTV serotype 8 (BTV-8) is of epidemiological importance in Central Europe and has caused recent major Bluetongue Disease outbreaks. The virus is transmitted by certain midges of the genus Culicoides and can also be spread by contaminated needles and surgical equipment.

* Depending on PCR-Cycler. See Handbook for detailed information.
** Pool recommendations and regulations may vary between countries.

Characteristics / Technical Specifications

• Product contents: Master Mix, Positive Control, Negative Control, Handbook
• Pathogen type: Virus
• Product type: Ready-to-use assay
• Species: Ruminants
• Pathogen investigated: Bluetongue Virus (BTV)
• Technology: RT-qPCR
• Cat. No.: VT280465