Iron assay kit BBITK-HMM-0006

for researchpharmacologyhemoglobin

Product Details
Product Name: Serum Iron Content Assay Kit (Microplate Reader). Catalog No.: BBITK-HMM-0006. Testing Equipment: Microplate Reader. Matching: 96-well plate. Number of Testable Samples: 100 samples. Estimated Measurement Time: ~4 h (100 samples). Storage: Store at 4°C protected from light. Self-contained Reagents: CHCl3 (MW=119.39, CAS: 67-66-3); C2H4O2 (MW=60.05, CAS: 64-19-7). Detection Principle: Sodium sulfite reduces Fe3+ to Fe2+; Fe2+ forms a colored complex with 2,2'-bipyridine with characteristic absorption at 520 nm. Detection Method: 2,2'-Bipyridine Colorimetric Method. Detection Wavelength: 520 nm. Signal Response: Incremental. Standard: Iron Standard Solution. Reference Standard Curve: y = 1.716x + 0.0016 (R2 = 0.9999). Standard Linear Range: 0.025-0.3 µmol/mL. Detection Limit: 0.002 µmol/mL. Note: If absorbance exceeds the linear range, dilute serum/plasma appropriately before measurement; if below range, increase sample volume and adjust calculations. Reagent 2 has limited validity after preparation; a spare bottle is included. Each Reagent 2 bottle supports at least 60 determinations.

Background
Iron is an essential trace element and a core component of hemoglobin, myoglobin and multiple enzyme systems (e.g., cytochromes, catalase). Accurate serum iron measurement is critical in medical and biochemical research to assess iron metabolism, nutritional status and related pathologies. This kit uses a reliable colorimetric reaction compatible with microplate readers to enable reproducible, high-throughput quantification suitable for research laboratories.

Product Features

• Established 2,2'-Bipyridine colorimetric method for iron quantification.
• Designed for microplate readers to enable high-throughput analysis.
• Includes matched 96-well plate for streamlined setup.
• Wide linear range (0.025–0.3 µmol/mL) to minimize repeat dilutions.
• Low detection limit (0.002 µmol/mL) for sensitive measurements.
• Clear incremental absorbance response at 520 nm for straightforward readout.
• Pre-formulated reagents with a spare bottle for Reagent 2; each bottle supports ≥60 samples.
• Estimated throughput: ~100 samples in ~4 hours.
• Storage at 4°C, protected from light.

Product Advantages

• High accuracy with validated reference standard curve (R² = 0.9999).
• Protocol suitable for trained researchers and laboratory technicians.
• Cost-effective for medium-to-high throughput studies (kit supports 100 tests).
• Flexible sample handling: dilution or adjusted sample volume for out-of-range values.
• Standardized reagents and quality controls reduce inter-batch variability.
• Intended for research use across biochemistry, physiology, nutrition and pharmacology.

Technical Specifications

• Catalog No.: BBITK-HMM-0006
• Product Name: Serum Iron Content Assay Kit (Microplate Reader)
• Testing Equipment: Microplate Reader
• Matching Consumable: 96-well plate
• Testable Samples: 100 samples
• Estimated Measurement Time: ~4 h (100 samples)
• Storage: 4°C, protected from light
• Self-contained Reagents: CHCl3 (MW 119.39, CAS 67-66-3); C2H4O2 (MW 60.05, CAS 64-19-7)
• Detection Principle: Fe3+ reduced to Fe2+ by sodium sulfite; Fe2+ forms colored complex with 2,2'-bipyridine; absorbance at 520 nm
• Detection Method: 2,2'-Bipyridine colorimetric method
• Detection Wavelength: 520 nm
• Signal Response: Incremental
• Standard: Iron Standard Solution
• Reference Standard Curve: y = 1.716x + 0.0016 (R2 = 0.9999)
• Standard Linear Range: 0.025-0.3 µmol/mL
• Detection Limit: 0.002 µmol/mL
• Notes: Dilute samples above linear range or increase sample volume if below range. Reagent 2 has limited validity after preparation; spare bottle included. Each Reagent 2 bottle supports at least 60 determinations.