Product name Bursaphelenchus xylophilus qPCR Kit PT-QCY-0012
Catalog No. PT-QCY-0012
ApplicationDetection of Bursaphelenchus xylophilus in plant tissue, wood samples and potential insect vectors. Intended for diagnostic and phytosanitary testing in laboratory environments.
Test methodReal-time quantitative PCR (qPCR) using probe-based chemistry for specific detection and quantification.
Kit composition- qPCR master mix: 500 μL
- Dilution buffer: 1 mL
- qPCR primer-probe mix: 100 μL
- qPCR positive control (1×10^7 copies/μL): 50 μL
Features- Reagents supplied ready-to-use; users provide extracted DNA and consumables.
- Probe-based assay format for target-specific amplification and detection.
- Positive control included to verify extraction and amplification workflow.
- Suitable for plant, wood and insect vector testing workflows.
Usage and protocol summary- Follow supplied datasheet for reaction setup; typical reaction volume 20 μL.
- Recommended thermal profile: initial denaturation followed by 40 amplification cycles (refer to datasheet).
- Include positive and negative controls for result validation; quantification via standard curve if required.
StorageStore all components at -20°C. Avoid repeated freeze–thaw cycles. Thaw on ice and mix thoroughly before use.
Technical specifications- Catalog No.: PT-QCY-0012
- Available quantity: 50T
- Intended use: Detection of Bursaphelenchus xylophilus in plant and insect samples
- Test method: Real-time qPCR (probe-based)
- Storage conditions: -20°C; avoid repeated freeze-thaw cycles