Magnetic bead-based reagent GST

for drug discoveryfor protein purificationfor research

Product Details
Product name: GST Fusion Protein Purification Kit
Catalog No.: SM-HMM-0062
Provided quantity: 5 mL (10% v/v) magnetic glutathione beads. Average particle size: 30–150 µm. Preservation fluid: 20% ethanol. Storage: short-term 4°C–30°C, long-term 4°C–8°C recommended.

Background
GST-tagged recombinant proteins are widely used in research, structural biology and drug discovery. Cell lysates and in vitro expression mixtures contain nucleic acids, host proteins and metabolites that complicate downstream assays. Magnetic glutathione-functionalized beads enable specific capture of GST fusion proteins and allow one-step purification by magnetic separation without chromatography equipment, facilitating routine lab workflows and small-scale production.

Product Features

• Magnetic separation format for rapid bead recovery using a magnetic stand; typical purification cycle ~1–2 hours.
• Compatible sample types: E. coli, insect cell and mammalian cell lysates, and in vitro transcription–translation supernatants.
• Optimized bead size (30–150 µm) balancing surface area and magnetic responsiveness for efficient binding and fast recovery.
• Designed for native purification conditions to preserve protein structure and activity.
• Storage-stable in 20% ethanol; shelf life ≥12 months under recommended conditions.

Product Advantages

• One-step purification yields high purity (typically ~80–90% depending on expression and sample complexity).
• Simplified workflow (load, wash, elute) without FPLC or column hardware—suitable for users with limited chromatography experience.
• High ligand density for strong binding capacity across milliliter-scale and small-batch preparations.
• Mild conditions and optimized washing reduce non-specific adsorption and mechanical loss; typical recoveries >70% under standard protocols.
• Cost-effective for R&D and small-scale production—kit format reduces waste from bulk purchases.

Related Products

• Glutathione-modified resins for column/FPLC workflows (industrial-scale purification).
• Disposable gravity-flow columns compatible with GST purification.
• Reduced glutathione elution buffers sold separately.
• Protease kits (enterokinase, thrombin) for tag removal when native target protein is required.
• GST-specific antibody kits for downstream verification (Western blot, ELISA).

Technical specifications

• Catalog No.: SM-HMM-0062
• Provided quantity: 5 mL (10% v/v)
• Storage: 4°C–30°C (long-term 4°C–8°C recommended)
• Average particle size: 30–150 µm
• Preservation fluid: 20% ethanol
• Concentration: 10% (v/v)
• Applications: Isolation and purification of GST fusion proteins; enzyme activity assays; antigen preparation; pull-down interaction studies; small-batch functional protein production.
• Intended use: For research use only. Not for clinical or diagnostic use.