Agarose gel reagent Streptavidin

microspherefor cell isolationfor immunoanalysis

Product details

• Product Name: Streptavidin (10–30 µm)
• Catalog No.: SM-HMM-0027
• Description: Agarose beads covalently coated with streptavidin for capture of biotinylated molecules in immunoprecipitation, ELISA, cell sorting, protein–DNA hybridization and sequencing workflows.
• Storage: 2–8°C (short-term ambient transport acceptable)
• Shelf Life: 2 years
• Average Particle Size: 10–30 µm
• Shell: Agarose
• Preservation Fluid: 1×PBS with 0.1% (w/v) BSA, 0.1% (v/v) proclin-300
• Concentration: 20% (v/v)

Background
Streptavidin is a tetrameric protein from Streptomyces avidinii with exceptionally high affinity for biotin (Kd ≈ 10⁻¹⁴ M). This non-covalent interaction is widely used in molecular biology for immobilization, detection and purification tasks.
The product's 10–30 µm particle size provides a balance between surface area and handling properties, suitable for solid-phase assays, SPR, microplate-based assays and manual or automated separation workflows.
Streptavidin's neutral isoelectric point (pI ≈ 5–6) helps reduce nonspecific interactions in complex samples, improving assay specificity.

Product Features

• Affinity for biotin: Kd ≈ 10⁻¹⁴ M for stable capture under stringent conditions
• Thermal tolerance: retains activity at elevated temperatures (up to ~80°C) for repeated thermal cycles
• Chemical compatibility: tolerant to common detergents (SDS, Triton X‑100), organic solvents and chaotropes
• Low nonspecific binding: neutral surface charge minimizes background in sensitive assays
• Conjugation-ready: accessible primary amines for dye/enzyme labeling or magnetic coupling

Product Advantages

• Reduced reagent use due to high binding capacity per unit
• Rapid binding kinetics (ka ≈ 10⁷ M⁻¹s⁻¹) to accelerate assay workflows
• Compatible with manual and high-throughput automated systems
• Stable shelf life of 24 months when stored at 2–8°C

Related Products

• Biotinylated antibodies for sandwich ELISA
• Magnetic streptavidin beads for rapid separation and cell isolation
• Fluorescent streptavidin conjugates for microscopy and flow cytometry
• Biotinylated nucleic acids or peptides for immobilization
• Pre-blocked streptavidin variants with reduced nonspecific binding

Technical specifications

• Product Name: Streptavidin (10–30 µm)
• Catalog No.: SM-HMM-0027
• Description: Streptavidin-coated agarose beads for capture of biotinylated molecules in IP, ELISA, cell sorting, hybridization and sequencing applications
• Storage: 2–8°C (short-term ambient transportation/storage acceptable)
• Shelf Life: 2 years
• Average Particle Size: 10–30 µm
• Shell: Agarose
• Preservation Fluid: 1×PBS with 0.1% (w/v) BSA, 0.1% (v/v) proclin-300
• Concentration: 20% (v/v)