Magnetic bead-based reagent SD-HMM-0003

solutionenzymeproteinase K

Product Details
Product Name: Tissue DNA Extraction Kit (20 rxns)
Catalog No.: SD-HMM-0003
Description: Rapid, magnetic bead–based kit for isolation of genomic DNA from fresh animal or human tissue samples. Samples are lysed and digested with Proteinase K; genomic DNA binds to magnetic beads and is recovered after wash and elution steps, yielding high-purity DNA suitable for molecular assays.
Inspection Principle: Proteinase K digestion followed by magnetic bead-based adsorption and wash/elution enables removal of proteins, lipids and RNA to produce intact genomic DNA for downstream analysis.
Features: High-throughput compatible; automated processing up to 96 samples in ~40 min. Typical yield 1–5 µg DNA per mg tissue. Purity A260/A280 1.8–1.9; A260/A230 ≥ 1.9. Storage 2–35°C. Shelf life 1 year.

Product Composition

• Magnetic Bead Suspension: 0.4 mL
• Lysis Solution (Buffer LB): 4.6 mL
• Wash Solution 1 (Buffer W1): 12 mL (add 12 mL isopropyl alcohol before first use)
• Elution Solution (Buffer EB): 2 mL
• Proteinase K: 0.4 mL
• Tissue Digestion Solution (Buffer TD): 4 mL

Product Overview

Product Introduction
This kit combines a robust Proteinase K-based digestion with a magnetic bead purification workflow to efficiently lyse diverse tissue types (including fibrous or fatty samples) and separate high-molecular-weight genomic DNA from contaminants. The protocol minimizes shearing and delivers DNA compatible with demanding downstream methods.

Product Application

• Genotyping and mutation analysis from animal model tissues (mouse, rat, etc.)
• Molecular analysis of clinical biopsy specimens (e.g., tumor tissue)
• Genetic screening in livestock and veterinary diagnostics
• Wildlife conservation genetics and forensics

Product Advantages

• Powerful lysis: effective across a wide range of tissue types, including fibrous and fatty samples
• High DNA integrity: minimizes fragmentation for long-fragment applications
• User-friendly protocol: clear steps from homogenization to elution
• Consistent yields: reproducible results across sample types; automation-ready

Technical Specifications

• Catalog number: SD-HMM-0003
• Commercial name: Tissue DNA Extraction Kit
• Pack size: 20 rxns
• Throughput: up to 96 samples in ~40 minutes (automation compatible)
• Expected yield: ~1–5 µg DNA per mg tissue (dependent on sample)
• Purity: A260/A280 1.8–1.9; A260/A230 ≥ 1.9
• Key components and volumes: Magnetic Bead Suspension 0.4 mL; Lysis Solution 4.6 mL; Wash Solution 1 (12 mL; add 12 mL isopropyl alcohol before first use); Elution Solution 2 mL; Proteinase K 0.4 mL; Tissue Digestion Solution 4 mL
• Storage conditions: 2–35°C
• Shelf life: 1 year
• Suitable downstream applications: PCR, real-time PCR, SNP genotyping, STR genotyping, next-generation sequencing, pharmacogenomics research
• Intended use: For research use only; not for clinical diagnostic use