IgG reagent SM-HMM-0030

magnetic bead-basedantibodyfor protein extraction

Product Details
Product Name: Protein A/G Immunoprecipitation Kit
Catalog No.: SM-HMM-0030
Description: Protein A/G magnetic beads utilize nanosurface technology to encapsulate Protein A/G proteins in high density onto the surface of superparamagnetic microspheres. The product has more antibody binding sites, uses fewer beads, and has a low non-specific binding rate, allowing for easy and efficient immunoprecipitation experiments. The beads can bind more than 300 µg of Human IgG per mL, and only 25 µL of beads are required for a single precipitation reaction. The large specific surface area of micron-sized beads dramatically reduces the equilibration time required for antibody and antigen adsorption, with antibody adsorption completed within 15 minutes and antigen precipitation within 30 minutes. The short operation time avoids the hydrolysis of the target protein caused by prolonged operation and ensures the activity of the target protein and the integrity of the protein complex.

Features

• High efficiency, high output, low consumption.
• Flexible and easy operation.
• Reliable and reproducible results.
• Low non-specific adsorption.

Storage
Stable at 2-8°C (can be stored or transported at room temperature for short periods of time)

Shelf Life
2 years

Applications
This product can be widely used for the immunoprecipitation reaction of antigens in cell lysates, cell secretion supernatants, serum, ascites and other samples.

Background
Immunoprecipitation (IP) is a cornerstone technique in molecular biology and biochemistry, widely used to isolate and purify specific antigens, protein complexes, or target proteins from complex biological samples. It relies on the specific binding between antibodies and antigens to achieve selective enrichment, enabling downstream analyses such as Western blotting, mass spectrometry, and protein-protein interaction studies.
Traditional IP methods often use agarose or sepharose beads as carriers for antibodies, but they face limitations including low antibody binding capacity, long incubation times, high non-specific adsorption, and cumbersome centrifugation steps. These drawbacks can lead to reduced recovery of target proteins, compromised protein activity, and inconsistent experimental results.
With advancements in nanotechnology and material science, magnetic bead-based IP kits have emerged as a revolutionary alternative. Protein A/G magnetic beads combine the high affinity of Protein A and Protein G for antibodies from multiple species (including human, mouse, rabbit, and goat) with the convenience of magnetic separation. This integration addresses the shortcomings of traditional methods, making IP experiments more efficient, reproducible, and user-friendly.

Product Features

• High-density Protein A/G coating using nanosurface technology to maximize antibody binding sites.
• Exceptional antibody binding capacity: binds over 300 µg Human IgG per mL of beads.
• Rapid reaction kinetics: antibody adsorption completed in ~15 minutes; antigen precipitation in ~30 minutes.
• Low non-specific binding due to optimized bead surface.
• Minimal bead usage: only 25 µL beads required per precipitation reaction.
• Gentle magnetic separation eliminates centrifugation, preserving protein complex integrity.
• Compatible with multiple sample types: cell lysates, secretions, serum, ascites, and more.
• Stable storage at 2-8°C with short-term room temperature tolerance.

Product Advantages

• High efficiency and productivity with short incubation times and high binding capacity.
• Reliable and reproducible results with low non-specific binding.
• User-friendly workflow via magnetic separation suitable for varied skill levels.
• Cost-effective due to low bead consumption and 2-year shelf life.
• Preserves protein activity by minimizing operation time and mechanical stress.
• Versatile applications including co-immunoprecipitation (Co-IP), chromatin immunoprecipitation (ChIP), and pull-down assays.

Technical Specifications

• Product name: Protein A/G Immunoprecipitation Kit
• Catalog number: SM-HMM-0030
• Bead type: Protein A/G coated superparamagnetic microspheres (nanosurface technology)
• Antibody binding capacity: >300 µg Human IgG per mL of beads
• Bead usage per reaction: 25 µL per precipitation reaction
• Antibody adsorption time: ~15 minutes
• Antigen precipitation time: ~30 minutes
• Compatible sample types: cell lysates, cell secretion supernatants, serum, ascites and similar biological samples
• Storage: 2-8°C (short-term room temperature acceptable)
• Shelf life: 2 years