Microsphere reagent SM-HMM-0039

antibodymagnetic bead-basedfor cell culture

Product Details
Product Name: Human CD3/CD28 T Cell Activation Magnetic Beads
Catalog No.: SM-HMM-0039
Description: Magnetic beads (5 µm diameter) covalently coated with anti-CD3 and anti-CD28 antibodies for rapid activation and expansion of human T cells without feeder cells or antigen. Beads are super-paramagnetic and can be separated using a magnetic rack. Recombinant human IL-2 may be added to enhance expansion.
Storage: Store at 2-8°C, do not freeze.
Concentration: 1 x 10^8 beads/mL
Applications: For use with PBMCs or isolated T cell subsets (CD3+, CD4+, CD8+).

Product Introduction
These beads provide a defined, dual-signal stimulus by engaging CD3 (TCR-like signal) and CD28 (co-stimulation) on T cells. The surface presentation of both antibodies on 5 µm beads mimics antigen-presenting cell interactions, enabling robust and sustained T cell activation and proliferation suitable for immunology research, CAR-T development, and ex vivo expansion workflows.

Product Application

• In vitro activation and expansion of human T cells for research
• CAR-T cell and adoptive cell therapy development and manufacturing research
• Studies of T cell signaling, differentiation, memory formation and exhaustion
• Antigen-specific or polyclonal T cell culture protocols

Product Advantages

• Dual-signal activation: Presents both Signal 1 (CD3) and Signal 2 (CD28) on the bead surface for physiological co-stimulation.
• Magnetic separation: Super-paramagnetic beads are easily removed after activation with a magnetic rack to stop stimulation.
• Reproducible stimulus: Defined antibody coating yields consistent activation across experiments versus variable APCs.
• High expansion potential: Supports vigorous proliferation to generate large cell numbers for assays or downstream applications.

Characteristics / Technical Specifications

• Bead type: inert super-paramagnetic beads
• Bead diameter: 5 µm
• Surface coupling: covalently coupled with anti-CD3 and anti-CD28 antibodies
• Concentration: 1 x 10^8 beads/mL
• Intended sample types: PBMCs or isolated T cell subsets (CD3+, CD4+, CD8+)
• Recommended supplement: Recombinant human IL-2 may be used to promote expansion
• Removal method: Magnetic rack for bead separation after activation
• Storage conditions: 2-8°C, do not freeze
• Intended use: For research use only, not for clinical use