Proteinase K reagent SD-HMM-0007

magnetic bead-basedbuffer solutionenzyme

Product Name
Plant DNA Extraction Kit (20 rxns)

Catalog No.
SD-HMM-0007

Description
Plant DNA Kit is suitable for fast and efficient extraction of DNA from plant samples using superparamagnetic microspheres. The pre-made buffer can be directly used for extraction, or can be adjusted according to the amount of sample added. This product can be used for manual extraction of a small amount of samples, and is also suitable for high-throughput operations with automated workstations. The extracted product can be used for enzyme digestion, PCR amplification, detection and other follow-up experiments.

Features

• Alcohol free system makes lysis more efficient.
• Suitable for different tissue samples of plants especially complicated samples such as seed.
• Room temperature lysis to make DNA more stable, no need water bath incubation.

Storage
At 2-35°C

Shelf Life
1 year

Product Composition
Magnetic Bead Suspension (Beaver Beads): 0.3 mL
Lysis solution (Buffer LB): 12 mL
RNase A: 0.3 mL
Binding solution (Buffer BB): 12 mL
Wash 1 (Buffer W1): 24 mL
Wash Solution 2 (Buffer W2): 4.8 mL (Note: Adding 19.2 mL of anhydrous ethanol before first use)
Elution solution (Buffer EB): 2 mL
Proteinase K solution (ProteinaseK): 0.3 mL

Product Introduction
Plant tissues are notoriously difficult for DNA extraction due to high levels of complex polysaccharides, polyphenols, and secondary metabolites that co-precipitate with or inhibit DNA. This kit uses a tailored lysis buffer system to effectively disrupt tough plant cell walls while neutralizing these inhibitory compounds. The protocol often involves a selective precipitation or column-based purification step to yield high-molecular-weight DNA that is clear, colorless, and ready for enzymatic reactions.

Product Application

• Plant genotyping, marker-assisted selection (MAS), and genetic mapping.
• DNA barcoding, phylogenetic studies, and biodiversity assessment.
• Detection of genetically modified organisms (GMOs) or plant pathogens.
• Preparation of DNA for whole-genome sequencing or targeted NGS panels.

Product Advantages

• Polysaccharide & Polyphenol Removal: reduces common plant-derived PCR inhibitors.
• Broad Compatibility: effective for a wide range of species, from soft leafy tissues to seeds, woody stems, and polysaccharide-rich tubers.
• High Yield: optimized to recover ample DNA from small amounts of starting material.
• Simple & Fast: streamlined protocol reduces hands-on time compared to traditional CTAB methods.

Technical specifications

• Catalog Number: SD-HMM-0007
• Pack size: 20 rxns
• Storage conditions: 2-35°C
• Shelf life: 1 year
• Extraction method: magnetic bead (superparamagnetic microspheres)
• System: alcohol-free lysis; room temperature lysis (no water bath required)
• Suitable sample types: various plant tissues including seeds, leaves, woody stems, tubers
• Downstream compatibility: enzyme digestion, PCR amplification, sequencing and other molecular applications
• Notable kit components and volumes: Magnetic Bead Suspension 0.3 mL; Buffer LB 12 mL; RNase A 0.3 mL; Buffer BB 12 mL; Buffer W1 24 mL; Buffer W2 4.8 mL (requires addition of 19.2 mL anhydrous ethanol before first use); Buffer EB 2 mL; Proteinase K 0.3 mL