Magnetic bead-based reagent SD-HMM-0005

lysozymebuffer solutionproteinase K

Product Overview
Product Name: Bacterial Genomic DNA Extraction Kit (10 rxns)
Catalog No.: SD-HMM-0005
Method: Magnetic beads-based genomic DNA extraction
Short description: Rapid extraction of high-purity bacterial genomic DNA from Gram-positive and Gram-negative samples, compatible with downstream PCR and sequencing.

Key Features

• Fast workflow: Typical protocol duration ~40 minutes; some colony-to-DNA workflows <30 minutes.
• High purity: Minimal RNA and protein carryover.
• Broad applicability: Validated for diverse bacterial species.
• No toxic solvents: Phenol/chloroform-free procedure.

Applications

• 16S rRNA sequencing for bacterial identification.
• PCR detection of pathogens and resistance genes.
• Preparation of genomic templates for cloning and functional studies.
• Metagenomic analysis from isolated colonies.

Kit Composition

• Beaver Beads — 0.2 mL
• Buffer AL — 3 mL
• Buffer LB — 2.5 mL
• Buffer W1 — 6 mL
• Buffer EB — 2 mL
• Proteinase K — 0.2 mL
• Lysozyme — 36 mg

Technical Specifications

• Catalog No.: SD-HMM-0005
• Commercial name: Bacterial Genomic DNA Extraction Kit
• Pack size: 10 rxns
• Extraction method: Magnetic beads-based genomic DNA extraction
• Sample compatibility: Gram-positive and Gram-negative bacteria
• Typical experiment duration: ~40 minutes
• Storage conditions: 2-35°C
• Shelf life: 2 years

Advantages

• Reliable PCR-ready DNA: Purified DNA is free of common inhibitors.
• Efficient for a broad bacterial range including tough cell walls after enzymatic lysis.
• Streamlined protocol suitable for routine screening and small-scale genomics workflows.

Storage & Shelf Life
Store at 2-35°C. Shelf life: 2 years.

Note
For research use only, not for clinical use.