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Protease reagent ETR-HMM-0004
enzymefor biochemistryfor research

Protease reagent - ETR-HMM-0004 - Alta DiagnoTech - enzyme / for biochemistry / for research

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Characteristics

Type: protease, enzyme
Applications: for biochemistry, for research, for spectrophotometer
Tested parameter: for enzymes
Method: colorimetric, enzymatic
Other characteristics: assayed

Description

Overview
Neutral protease (NP) is an enzyme that hydrolyzes peptide bonds under neutral conditions to yield peptides and free amino acids. Its substrate specificity and mild reaction conditions make it suitable for analytical activity measurement in laboratory workflows.

Principle
The reagent enables measurement of NP activity by catalyzing the hydrolysis of casein to release tyrosine under neutral conditions. Tyrosine reduces phosphomolybdate compounds to form a tungsten-blue complex in alkaline medium. The chromophore has a characteristic absorbance at 680 nm; NP activity is determined by the change in absorbance (ΔA) using the Folin detection method and a tyrosine standard curve.

Product details

Product name: Neutral Protease (NP) Activity Assay Reagent (Microplate Reader)
Catalog No.: ETR-HMM-0004
Applicable instruments: Microplate reader
Number of testable samples: 50 samples
Format / matching: 96-well plate
Detection time: 5 h (for 50 samples)
Detection method: Folin method
Spectral parameters: measurement at 680 nm
Signal response: incremental
Standard: Tyrosine
Reference standard equation: y = 0.8108x + 0.0072 (R2 = 0.9999)
Standard linear range: 0.05–1.2 μmol/mL

Technical specifications

Sample throughput: designed for batch processing of up to 50 samples per kit using a 96-well plate
Sensitivity: linear response within the specified tyrosine range
Readout: absorbance at 680 nm
Compatibility: standard microplate readers that measure 680 nm

Procedure notes

If the measured ΔA is small, extend the first reaction step to 15–25 minutes and adjust calculations accordingly
Prepare standard curve with provided tyrosine standard; verify linearity before sample measurement
Run appropriate blanks and controls to account for matrix effects

Storage and safety

Store reagents according to manufacturer instructions (refer to label) and avoid repeated freeze–thaw cycles
For in vitro laboratory use only; follow institutional biosafety and waste‑disposal procedures
Observe standard handling precautions for biochemical reagents

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