OverviewMagnetic bead-based cell isolation uses immunomagnetic beads to label target cells; bead-bound and unbound cells are separated by an external magnetic field. This kit provides a negative-selection protocol for enrichment of human CD34+ cells. Positive-selection workflows are available in alternate products to suit different experimental needs.
Features- One-step enrichment up to 10-fold concentration.
- Two-step enrichment up to 30-fold concentration.
- CD34+ cell enrichment in approximately 30 minutes.
- Column-free workflow; use with a magnetic separator.
Packaging / QuantitiesStorageStore at 2-8°C. Do not freeze.
ApplicationsDesigned for enrichment of CD34+ cells from human cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) for research applications.
Additional InformationFor research use only. Not for clinical or diagnostic use.
Technical specifications- Product Name: Human CD34+ Cell Enrichment Kit (Negative Selection)
- Catalog No.: SM-HMM-0046
- Principle: Immunomagnetic bead-based cell isolation using an external magnetic field to separate bead-bound and unbound cells.
- Separation Technologies: Negative selection (this kit); positive selection available in alternate kits.
- Enrichment Performance: One-step enrichment up to 10-fold; two-step enrichment up to 30-fold.
- Processing Time: CD34+ enrichment in ~30 minutes.
- Column Requirement: Column-free; use with a compatible magnetic separator.
- Storage Conditions: 2-8°C; do not freeze.
- Intended Samples: Human cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC).
- Packaging Options: 5×10^8 cells and 1×10^9 cells.