Solution reagent kit fE3

for antibodieschemiluminescence

The kit has been designed for the quantitative determination of Free Estriol (fE3) in human serum. The method can be used for samples over the range of 0.04-9.00ng/mL. The test has to be performed Taizhou ZECEN Biotech Co., Ltd. model CIA 600, CIA 600Plus, CIA 1200, CIA 1200M, CIA 1800, CIA 2800 and POClia 8, POClia minus, POClia plus, POClia auto automatic chemiluminescence analyzer. Shenzhen IncreCare Biotech Co., Ltd model Shine i1900Shine i2900 automatic chemiluminescence analyzer. PRINCIPLE OF THE TEST Competing method: fE3 in samples, calibrators or positive control, competes with the fE3 labeled by FITC to bind the fE3 monoclonal antibody labeled by AP and form Ab-Ag complex. Then add the anti-FITC magnetic beads. Through the specific binding of anti-FITC antibody with FITC, the Ag-Ab complex will be occupied by the magnetic particles. The entire complex will be captured by the applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through s1699408931533oftware by comparing the luminous intensity with the cutoff value. Storage and Stability ⚫ Opened: Stable for 4 weeks. To ensure best kit’s performance, it is recommended to place opened kits in the refrigerator if it’s not going to be used on board during next 12 hours.