Collagen reagent kit DR1032

for antigens

In human liver, Type IV Collagen is highly upregulated with a 14-fold increase in liver cirrhosis, which is the highest relative increase among all collagen types. In Hepatitis C-related fibrosis, Type IV Collagen immunoreactivity displayed a steep rise with progression of fibrotic changes and its expression was found to be a useful discriminator between early and late stages of fibrosis. Antigens related to the 7S and NCI domains of Type IV Collagen have been evaluated as noninvasive biomarkers of chronic liver disease. Expression of Type IV Collagen in the space of Disse results in formation of a perisinusoidal basement membrane in liver fibrosis. [INTENDED USE] The kit has been designed for the quantitative determination of Collagen Ⅳ (CⅣ) in human serum. The method can be used for samples over the range of 20-2400 ng/mL. [SUMMARY AND EXPLANATION OF THE TEST] Collagen Ⅳ (hereinafter referred to as CⅣ) quantitative detection kit (CLIA) is used for the in vitro quantitative detection of CⅣ concentration in serum. It is an indicator which reflects collagen production rather than degradation, and the degree of liver fibrosis. CⅣ plays animportant role in diagnosis of liver fibrosis and cirrhosis. CⅣ in normal liver is mainly in the base film of blood vessels and bile ducts, and there is no significant deposit in the hepatic sinusoid. When liver fibrosis occurs, Fiber hyperplasia of CⅣ may occur the earliest with high conversion rate, a large number of deposition, and finally forms a complete basement membrane with laminin through continuous deposition. It is now recognized as an indicator which reflects collagen production rather than degradation,