Tumour detection detection kit

for tumor markersfor antigensserum

The kit has been designed for the quantitative determination of Total Prostate Specific Antigen (t-PSA) in human serum. The method can be used for samples over the range of 0.02~280.0 ng/mL. The test has to be performed Taizhou ZECEN Biotech Co., Ltd. model CIA 600, CIA 600Plus, CIA 1200, CIA 1200M, CIA 1800, CIA 2800 and POClia 8, POClia minus, POClia plus, POClia auto automatic chemiluminescence analyzer. Product Description PRINCIPLE OF THE TEST Sandwich method： t-PSA Antibody labeled by FITC and t-PSA antibody pair labeled by AP bind with the t-PSA antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Then the entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists. SPECIMEN COLLECTION AND PREPARATION Collect samples using standard procedures. Avoid repeated freezing and thawing cycles, stored samples should be thoroughly mixed prior to use (Vortex mixer).