Staining solution reagent May Grünwald Giemsa

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 35 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Graduated cylinder Application The method of choice for differentiating cell types and highlighting parasites on tissue sections; particularly indicated for lymphohematopoietic tissue. This stain is often used for identifying endothelial reticulum. Result Nuclei blue Basophilic cytoplasm from sky blue to dark blue Acidophilic cytoplast pink Bacteria blue Product for the préparation of cyto-histological samples for optical microscopy. Recommended method to differentiate cell types and to reveal parasites in tissue sections. Especially useful for lymphopoietic tissue. This stain is often used to demonstrate endothélial réticulum. PRINCIPLE - - May Grunwald solution, consisting of eosin-methylene blue, stains nudei blue and basophil cytoplasm pinkish red. - - Giemsa solution, a complex consisting of methylene blue chloride, eosin-methylene blue and azuré II eosinate, improves the intensity of nuclear staining and the capacity to show selectively cellular structures. To appreciate results always remember two factors: pH of washing water and dilution buffer hâve a strong influence on final colour chart; intensity of stain may vary according to différentiation time.