Staining solution reagent Perls

for histologyfor cytology

Minimum number of tests that can be performed 72 Completion time 35 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment 50 ml vertical histology jar, graduated cylinder and glass rod Application Method indicated for viewing reactive ferric iron on tissue sections, blood smears and bone marrow smears. Specificity - the Perls reaction does not show all the iron present in the tissue: iron bound to hemoglobin, malaria pigment, ferritin, pigments deriving from the use of acid formalin and ferrous iron does not react. Result Reactive ferric iron blue Nuclei red Product for the préparation of cyto-histological samples for optical microscopy. To show reactive ferrie iron in tissue sections (method 1) and for haematology and cytology (method 2). PRINCIPLE Potassium ferrocyanide reacts with ferrie ions of hemosiderin in acid environment to form a coloured sait: Prussian blue. Reaction takes place in ionic form as follows: 4 Fe~~ 3K4Fe(CN)6 = Fe4(Fe(CN)6h+ 12 K*