Staining solution reagent P.A.S.M.

for cytologyfor histology

Product for the preparation of cyto-histological samples for optical microscopy. To show argyrophilic elements and mucopolysaccharides (basal membranes, mycetes, bacteria etc.) in tissue sections. Recommended method to examine basal membrane in renal biopsy. PRINCIPLE Periodic acid reacts with glycolic and glycoaminic groups in mucopolysaccharide chain oxidising them to aldehydic groups and thus breaking the chain itself. These newly formed aldehydic groups reduce silver chloride, which is part of the silvermethenamine complex, to metallic silver and make it visible. WARNING For good results, follow these rules: - Always use excellent and chlorine-free distilled or deionized water. - Use only perfectly clean glassware. - As in all reactions with silver salts, it is essential to use perfectly clean glassware and excellent distilled or deionized water. Do not touch reagents containing silver salts with metallic objects (tweezers etc.) METHOD 1) Bring section to distilled water. 2) Put on the section 10 drops of reagent A: leave to act for 30 minutes. 3) Rinse in distilled water. 4) Prepare the incubation box and lay down the slide; pour 10 drops of reagent B into the vial, add 10 drops of reagent C and 10 drops of reagent D, shake and put the obtained solution on the section, close the incubation box and incubate in oven at 60° C for 30-40 minutes. 5) Take out the incubation box from the oven and check the tone of impregnation, if it is correct wait 5 minutes and rinse in distilled water. If it is weak, reincubate in the oven and check every 5 minutes. 6) Put on the section 10 drops of reagent E: leave to act 1 minute.